Anti-Phospho-TBC1D4-T642 Rabbit Polyclonal Antibody for WB, ELISA - O60343

Core Product Specifications and Parameters

Biological Background: TBC1D4 (AS160) Function and Localization

• TBC1D4, also known as AS160 (Akt substrate of 160 kDa), is a member of the TBC1 domain family and acts as a GTPase-activating protein (GAP) for RAB proteins RAB2A, RAB8A, RAB10, and RAB14.
• Isoform 2 of TBC1D4 promotes insulin-induced translocation of glucose transporter SLC2A4/GLUT4 to the plasma membrane, thereby increasing glucose uptake in responsive tissues like skeletal muscle and adipose tissue.
• The gene encodes multiple isoforms; isoform 1 is highly expressed in skeletal muscle and heart, while isoform 2 is widely expressed with strong presence in adrenal gland, thyroid, lung, kidney, colon, brain, and adipose tissue.
• Subcellular localization is predominantly cytoplasmic, consistent with its role in vesicular trafficking and signal transduction from the insulin receptor.
• TBC1D4 is regulated by phosphorylation at multiple sites, including Thr642, which is mediated by Akt/protein kinase B downstream of insulin signaling, and is critical for its GAP activity and GLUT4 translocation.
• Expression is decreased in pancreatic islets from type 2 diabetes patients, linking TBC1D4 dysfunction to impaired glucose homeostasis; it is also up-regulated in T-cells from patients with atopic dermatitis, suggesting broader roles in immune modulation.
• The protein undergoes post-translational modifications including phosphorylation (key residues like Thr642), acetylation, and methylation, which fine-tune its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to Thr642, ensuring specificity for the phosphorylated form; always use phosphatase inhibitors during sample preparation to preserve phosphorylation status.
• For western blotting, the predicted molecular weight is 147 kDa; validate band specificity using non-phosphorylated peptide blocking or phosphatase-treated lysates.
• In ELISA applications, confirm coating efficiency and antibody titer with phosphorylated versus non-phosphorylated peptide controls.
• Species cross-reactivity with mouse has been reported; verify reactivity in your specific mouse strain or tissue, as expression levels may vary.
• Consider using this antibody to monitor insulin signaling pathways in models of insulin resistance or type 2 diabetes, combining with total TBC1D4 antibodies for normalization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and research laboratories, providing high-quality IVD raw materials and tailored sourcing solutions. This anti-phospho-TBC1D4-T642 antibody supports studies in insulin signaling and diabetes, complementing our portfolio of validated antibody pairs, bulk ancillary reagents, and rare target sourcing capabilities. Contact CamelBio today to streamline your workflow from concept to clinic with reliable, production-ready biomaterials.