Anti-Phospho-Tau-T231 Monoclonal Antibody for IF-P, ELISA - P10636

Core Product Specifications and Parameters

Biological Background: Tau Function and Localization

• Promotes microtubule assembly and stability, crucial for establishment and maintenance of neuronal polarity. Related references: PMID:21985311
• The C-terminal region binds axonal microtubules while the N-terminus interacts with neural plasma membrane components, acting as a linker between both. Related references: PMID:21985311 PMID:32961270
• Axonal polarity is predetermined by tau localization at the cell body domain defined by the centrosome.
• Multiple isoforms arise from alternative splicing; short isoforms allow cytoskeletal plasticity while longer isoforms stabilize microtubules.
• Subcellularly localizes to cytoplasm, cytosol, cell membrane, cytoskeleton, axonal and dendritic projections, and can be secreted.
• Expressed in neurons; the PNS-tau isoform is found in the peripheral nervous system, others in the central nervous system.
• Post-translational modifications include phosphorylation, acetylation, methylation, glycation, ubiquitination, and disulfide bonds; phosphorylation at T231 is disease-associated.
• Associated with Alzheimer's disease, Parkinsonism, frontotemporal dementia (FTDP-17), and other neurodegenerative disorders.

Experimental Guidance and Technical Tips

• As a phospho-specific antibody generated with a synthetic phosphorylated peptide immunogen, consider validating specificity by comparing signal to non-phosphorylated peptide or dephosphorylated samples.
• For immunofluorescence (IF), optimize permeabilization and fixation conditions to preserve the phosphorylated epitope; methanol fixation may enhance detection.
• In ELISA, use the phosphorylated peptide as a positive control and the non-phosphorylated counterpart as negative to confirm phospho-specificity.
• Given the low abundance and transient nature of phosphorylation events, include phosphatase inhibitors in lysis buffers if extending the antibody to other sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this anti-Phospho-Tau-T231 antibody to support Alzheimer's disease and neurodegeneration research. Our portfolio includes validated antibody pairs, recombinant proteins, and ancillary reagents, enabling seamless assay development. With capabilities in rare-target sourcing and bulk supply, we are committed to accelerating your projects from concept to clinic.