Anti-Phospho-Tau-T217 Rabbit Polyclonal Antibody (pAb) for WB, ELISA - P10636-5

Core Product Specifications and Parameters

Biological Background: Tau (Microtubule-Associated Protein Tau) Function and Localization

• Tau (Microtubule-associated protein tau) is a neuronal protein that stabilizes microtubules and is essential for axonal transport.
• Alternative splicing of the MAPT gene generates multiple isoforms; isoform Tau-C (UniProt P10636-5) is one of them, with a molecular weight of approximately 42.6 kDa.
• In neurodegenerative tauopathies such as Alzheimer's disease, tau becomes hyperphosphorylated at multiple sites, including T217, leading to aggregation into paired helical filaments and neurofibrillary tangles.
• The antibody specifically detects phosphorylation at threonine 217 (T217) of human tau, which is a pathological hallmark in Alzheimer's disease and other tauopathies.
• The protein is also known as neurofibrillary tangle protein or paired helical filament-tau (PHF-tau).

Experimental Guidance and Technical Tips

• Due to multiple isoforms and phosphorylation states, Tau can appear as bands between 32-45 kDa and 78-80 kDa in Western blot. Validate band identity using appropriate controls and phosphatase treatment experiments.
• For ELISA, consider using recombinant phosphorylated Tau peptide or cell lysates with known phosphorylation status to optimize coating and detection conditions.
• Pre-adsorption with non-phosphorylated peptide can help confirm phospho-specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials and technical services, supporting tauopathy research areas with high-quality antibodies like the Phospho-Tau-T217 Rabbit pAb. Our supply scope includes validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and customized rare-target raw-material sourcing to streamline diagnostic development from concept to clinic.