Anti-Phospho-Tau-T212 Rabbit Polyclonal Antibody for WB and ELISA - P10636

Core Product Specifications and Parameters

Biological Background: Tau (Phospho-T212) Function and Localization

• Microtubule-associated protein tau (MAPT) is a neuronal phosphoprotein that promotes microtubule assembly and stability. Multiple isoforms are generated by alternative splicing, with short isoforms allowing cytoskeletal plasticity and longer isoforms stabilizing microtubules.
• Tau binds both axonal microtubules via its C-terminus and neural plasma membrane components via its N-terminus, acting as a linker protein to establish and maintain neuronal polarity (PubMed:21985311, PubMed:32961270). Related references: PMID:21985311 PMID:32961270
• Located in the cytoplasm, cytosol, cell membrane, cytoskeleton, axons, and dendrites, and can be secreted. Predominantly expressed in neurons, with isoform PNS-tau in the peripheral nervous system and others in the central nervous system.
• Extensively modified by phosphorylation, acetylation, glycation, methylation, ubiquitination, and disulfide bond formation. Hyperphosphorylation, including at T212, is associated with Alzheimer’s disease and other tauopathies, leading to paired helical filament (PHF) formation and neurofibrillary tangles.
• Mutations and abnormal aggregation of tau are linked to neurodegenerative disorders such as Alzheimer disease, frontotemporal dementia with parkinsonism-17 (FTDP-17), and other tauopathies. T212 phosphorylation serves as a key pathological marker.
• Additional roles may include signaling and cellular protection, but its primary function is structural support of the neuronal cytoskeleton.
• UniProt keywords: 3D-structure, Acetylation, Alternative splicing, Alzheimer disease, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Direct protein sequencing, Disease variant, Disulfide bond, Glycation, Glycoprotein, Isopeptide bond, Membrane, Methylation, Microtubule, Neurodegeneration, Parkinsonism, Phosphoprotein, Proteomics identification, Reference proteome, Repeat, Secreted, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide surrounding T212; this antibody specifically recognizes tau phosphorylated at this residue. Use phosphatase inhibitors during sample preparation to preserve the phosphorylated state.
• For western blotting, the predicted molecular weight of full-length tau is ~79 kDa, but multiple isoforms and phosphorylation states may produce additional bands. Validate in your sample system.
• ELISA procedures can be applied for quantitative detection of phospho-Tau-T212 in cell lysates or biological fluids. Include appropriate positive and negative controls.
• Cross-reactivity with mouse and rat is reported, but verify in the relevant tissue type, as phosphorylation patterns may differ across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials for neurodegenerative disease research, including validated phospho-specific antibodies like this Anti-Phospho-Tau-T212 Rabbit pAb. Our supply chain supports validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets in the tauopathy biomarker field. From concept to clinic, CamelBio bridges the gap with reliable raw-material solutions and technical consultancy.