Anti-Phospho-Tau (T205) Monoclonal Antibody for WB, IHC-P, IF - P10636

Core Product Specifications and Parameters

Biological Background: Tau Function and Localization

• Microtubule-associated protein tau (MAPT) promotes microtubule assembly and stability, and is involved in neuronal polarity by linking microtubules and plasma membrane components. Related references: PMID:21985311 PMID:32961270
• Tau isoforms range from short isoforms that allow cytoskeletal plasticity to longer isoforms that stabilize microtubules.
• The protein is predominantly expressed in neurons, with specific isoforms in the central nervous system (CNS) and peripheral nervous system (PNS).
• Subcellular localization includes cytoplasm, cytosol, cytoskeleton, and cell projections such as axons and dendrites; tau is also secreted and associated with the cell membrane.
• Post-translational modifications such as phosphorylation (e.g., at T205), acetylation, methylation, glycation, and ubiquitination regulate tau function.
• Tau is implicated in neurodegenerative diseases, earning keywords like Alzheimer disease, Parkinsonism, and disease variant.
• Alternative splicing generates multiple isoforms, including tau-40 (2N4R) and PNS-tau.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around T205; the antibody is expected to specifically recognize Tau phosphorylated at Thr205.
• For western blotting, a band near 79 kDa is anticipated; consider using phosphatase-treated lysates to confirm phosphorylation-specific binding.
• In immunohistochemistry and immunofluorescence, antigen retrieval may be necessary; co-staining with a total Tau antibody can help assess phosphorylation status.
• Cross-reactivity with mouse and rat allows validation in rodent models, but confirm reactivity in your specific sample system.
• For ELISA applications, ensure the coating antigen presents the phosphorylated epitope appropriately.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with streamlined access to high-quality IVD raw materials, including validated phospho-specific antibodies like this anti-Phospho-Tau (T205) monoclonal antibody. Whether you need antibody pairs, optimized monoclonal or polyclonal antibodies, bulk reagents, or rare target sourcing, our one-stop platform supports your projects from concept to clinic. Rely on CamelBio as your procurement partner to accelerate neurodegeneration research and assay development.