Anti-Phospho-Tau (Ser416) Rabbit Polyclonal Antibody for WB, IHC, IF, ELISA - P10636

Core Product Specifications and Parameters

Biological Background: Tau Function and Localization

• Tau (microtubule-associated protein tau) is a neuronal phosphoprotein that promotes microtubule assembly and stability, and is critical for establishing and maintaining neuronal polarity. Related references: PMID:21985311
• The protein acts as a linker, with its C-terminus binding to axonal microtubules and the N-terminus interacting with neural plasma membrane components, thereby connecting the cytoskeleton to the cell membrane. Related references: PMID:21985311, PMID:32961270
• Multiple isoforms are generated by alternative splicing, with shorter isoforms providing cytoskeletal plasticity and longer isoforms contributing to microtubule stabilization.
• In neurons, Tau localizes to the cytoplasm, cytosol, cell membrane, and cytoskeleton, and is enriched in axons and dendrites; it can also be secreted.
• The protein is predominantly expressed in neurons, with the PNS-tau isoform specific to the peripheral nervous system and other isoforms found in the central nervous system.
• Tau undergoes extensive post-translational modifications, including phosphorylation, acetylation, methylation, glycation, and ubiquitination, which regulate its function and are implicated in neurodegeneration.
• Pathological hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles, a hallmark of Alzheimer disease and other tauopathies.

Experimental Guidance and Technical Tips

• This antibody is raised against a synthetic phosphopeptide corresponding to Ser416 of human Tau; it is expected to specifically detect Tau phosphorylated at Ser416 in human, mouse, and rat samples.
• For Western blot, a band near 79 kDa is anticipated, but multiple isoforms and post-translational modifications may produce additional bands; include appropriate phosphatase inhibitors during sample preparation.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), optimization of antigen retrieval and permeabilization conditions is recommended to ensure reliable detection in paraffin-embedded or fixed tissues.
• Validation in the relevant model system (e.g., neuronal cell lines, brain tissue) is advised, and parallel probing with a total Tau antibody can confirm phosphorylation-specific signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For tau-related neurodegeneration studies, we can source this phospho-Tau-Ser416 antibody along with additional reagents such as total Tau antibodies, recombinant Tau proteins, and Tau ELISA kits. Our one-stop procurement service streamlines access to rare-target raw materials, supporting your projects from concept to clinic.