Anti-Phospho-Tau-S404 Rabbit Monoclonal Antibody for WB and ELISA - P10636

Core Product Specifications and Parameters

Biological Background: Phospho-Tau Function and Localization

• Tau (microtubule-associated protein tau) is a key neuronal phosphoprotein that promotes microtubule assembly and stability, and is essential for establishing and maintaining neuronal polarity. Related references: PMID:21985311 PMID:32961270
• It acts as a linker protein: the C-terminal domain binds axonal microtubules, while the N-terminus interacts with neural plasma membrane components.
• Multiple isoforms are generated by alternative splicing; shorter isoforms enhance cytoskeletal plasticity, whereas longer isoforms predominantly stabilize microtubules.
• Tau localizes to the cytoplasm, cytosol, cell membrane, and cytoskeleton, and is found in axonal and dendritic projections; it can also be secreted.
• Expressed specifically in neurons, with distinct isoforms in the central and peripheral nervous systems.
• Extensively modified post-translationally—including phosphorylation at residues such as S404—and linked to neurodegenerative diseases like Alzheimer’s; keywords include Phosphoprotein, Alzheimer disease, and Neurodegeneration.
• The full-length protein migrates at approximately 79 kDa (UniProt: 78928 Da).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around S404, ensuring specific recognition of Tau phosphorylated at serine 404.
• For western blotting, consider using phosphatase inhibitors in lysis buffers and validate the antibody’s specificity with phosphatase treatment or non-phospho peptide blocking.
• The antibody reacts with human, mouse, and rat samples; it is recommended to confirm cross-reactivity in your specific experimental system.
• Standard ELISA protocols can be applied; include appropriate controls such as phosphorylated and non-phosphorylated peptides.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing platform for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets like phospho-Tau. With expertise in neurodegenerative disease research and diagnostic assay development, we assist partners from early R&D through to clinical translation.