Anti-Phospho-Tau (S396) Rabbit Monoclonal Antibody for WB, ELISA - P10636

Core Product Specifications and Parameters

Biological Background: Tau Function and Localization

• Tau (microtubule-associated protein tau) is encoded by the MAPT gene and exists in multiple isoforms due to alternative splicing.
• Promotes microtubule assembly and stability, and plays a critical role in establishing and maintaining neuronal polarity. Related references: PMID:21985311, PMID:32961270
• Functions as a linker between axonal microtubules and neural plasma membrane components via its distinct C- and N-terminal domains. Related references: PMID:21985311, PMID:32961270
• Predominantly localized in the cytoplasm, including the cytosol and cytoskeleton; also found at the cell membrane, in axons, dendrites, and can be secreted.
• Subject to various post-translational modifications including phosphorylation, acetylation, methylation, glycation, and ubiquitination, which regulate its function and aggregation.
• Expressed in neurons, with the PNS-tau isoform specific to the peripheral nervous system and other isoforms in the central nervous system.
• Hyperphosphorylated tau aggregates into paired helical filaments (PHF-tau) forming neurofibrillary tangles, a hallmark of Alzheimer's disease and other tauopathies.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphopeptide corresponding to Ser396 phosphorylation site of human tau; verify specificity using non-phosphorylated peptide or phosphatase-treated samples.
• For Western blot, the predicted molecular weight is ~79 kDa, but multiple isoforms and post-translational modifications may produce additional bands; optimize gel percentage and transfer conditions.
• In ELISA, consider using a phospho-specific detection system with appropriate controls to ensure signal specificity.
• Cross-reactivity has been observed with mouse tau; validate in the relevant sample system if using mouse models.

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CamelBio offers a comprehensive portfolio of IVD raw materials, including validated phospho-specific antibodies like this anti-Phospho-Tau (S396) rabbit monoclonal antibody for neurodegeneration research. Our one-stop sourcing platform provides access to antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw materials to support diagnostic assay development from concept to clinic. Partner with CamelBio for efficient procurement and tailored supply solutions.