Anti-Phospho-Syk-Y323 Polyclonal Antibody for WB, ELISA - P43405

Core Product Specifications and Parameters

Biological Background: Tyrosine-protein Kinase SYK Function and Localization

• Tyrosine-protein kinase SYK (also known as spleen tyrosine kinase or p72-Syk) is a non-receptor tyrosine kinase containing two N-terminal SH2 domains and a C-terminal kinase domain. It serves as a key mediator of signal transduction downstream of immunoreceptors such as the B-cell receptor (BCR).
• SYK regulates diverse processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation, and vascular development.
  Related references:
  PMID:12387735
  PMID:33782605
• Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2, and BLNK, linking receptor activation to calcium flux, MAPK, and NF-kB pathways.
  Related references:
  PMID:12456653
  PMID:15388330
  PMID:34634301
• In B-cells, SYK is essential for maturation at the pro-B to pre-B transition and for antigen-dependent BCR signaling.
  Related references:
  PMID:12456653
• Required for IL15-stimulated neutrophil phagocytosis, highlighting its role in innate immune cell function.
  Related references:
  PMID:15123770
• Subcellular localization includes the cell membrane, cytoplasm, and cytosol, consistent with its function in membrane-proximal signaling events.
• Widely expressed in hematopoietic cells, from pro-B and pre-B stages to mature plasma cells, and in neutrophils.
  Related references:
  PMID:8163536
• Annotated with UniProt keywords including kinase, SH2 domain, phosphoprotein, innate immunity, adaptive immunity, and host-virus interaction.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphorylated peptide surrounding Y323 of human Syk; it specifically recognizes the phosphorylated form.
• For Western blot, we recommend testing a range of antibody dilutions and including untreated versus phosphatase-treated lysates or peptide competition to confirm phospho-specificity.
• As a positive control, consider using lysates from B-cell lines (e.g., Ramos) treated with pervanadate or anti-IgM to induce Syk phosphorylation.
• For ELISA, use the phosphorylated immunogen peptide as the coating antigen; cross-adsorb against the non-phosphorylated peptide to ensure specificity.
• Avoid repeated freeze-thaw cycles and store the antibody at -20°C upon arrival; the product is shipped on ice bags.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw material partner, providing validated antibodies, matched antibody pairs, and supporting reagents for immunodiagnostic development. This phospho-specific anti-Syk antibody is part of our portfolio of signal transduction reagents for immune signaling research and clinical assay applications. We support customers from proof-of-concept to regulatory approval with customized sourcing and technical consulting.