Anti-Phospho-STK11-T189 Rabbit pAb for WB - Q15831

Core Product Specifications and Parameters

Biological Background: STK11 Function and Localization

• STK11 (also known as LKB1) is a serine/threonine-protein kinase that functions as a tumor suppressor and master upstream activator of AMPK family kinases, playing key roles in cell metabolism, cell polarity, apoptosis and DNA damage response.
  Related references:
  PMID:12805220 PMID:14517248 PMID:14976552

• It phosphorylates a wide range of downstream targets, including AMPK catalytic subunits (PRKAA1/2), BRSK1/2, MARK1-4, NUAK1/2, SIK1-3, SNRK, as well as non-AMPK proteins such as STRADA and PTEN.
  Related references:
  PMID:12805220 PMID:14517248 PMID:14976552 PMID:11430832 PMID:15987703

• STK11 is critical for establishing neuronal polarity by phosphorylating and activating BRSK1/BRSK2, which triggers axon specification.
  Related references:
  PMID:14976552

• In the DNA damage response, STK11 interacts with p53/TP53 and is recruited to the CDKN1A/WAF1 promoter to regulate transcription; it also modulates UV-induced DNA repair by controlling CDKN1A degradation via NUAK1.
  Related references:
  PMID:11430832 PMID:17108107 PMID:21317932 PMID:25329316

• The protein localizes to multiple subcellular compartments, including nucleus, cytoplasm, mitochondrion, and late endosome, enabling context-dependent signaling.

• Ubiquitously expressed, with highest levels in testis and fetal liver; it also plays a role in spermiogenesis.

• STK11 is subject to extensive post-translational modifications—including phosphorylation, acetylation, prenylation, and palmitoylation—that regulate its activity and subcellular targeting.

Experimental Guidance and Technical Tips

• As this antibody is raised against a phosphorylation site (T189), use phosphatase inhibitors during cell/tissue lysis to preserve the phospho-epitope for Western blotting.
• The antibody shows cross-reactivity with mouse and rat STK11; validate detection and specificity in your rodent model system before relying on quantitative data.
• For ELISA, consider competitive peptide assays (phosphorylated vs. non-phosphorylated peptide) to confirm phospho-specific binding.
• Include both phospho-specific and total STK11 controls in your experiments to distinguish phosphorylation-dependent signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering streamlined access to validated reagents for kinase signaling research, including phospho-specific antibodies, matched antibody pairs, and ancillary buffers. Whether you need bulk quantities of this anti-phospho-STK11-T189 antibody or require customized sourcing for rare specificity targets, our team helps bridge your development needs from concept to clinic. Reach out to explore how we can support your next diagnostic or research project.