Anti-Phospho-Stathmin 1 (Ser16) Rabbit Polyclonal Antibody for WB and ELISA - P16949

Core Product Specifications and Parameters

Biological Background: Stathmin 1 Function and Localization

• Stathmin 1 (also known as Oncoprotein 18, Op18, metablastin, LAP18, pp19, prosolin, PR22, pp17, and Lag) is a ubiquitous phosphoprotein that plays a key role in microtubule dynamics by promoting disassembly and preventing assembly of microtubules.
• The protein is a major regulatory component of the microtubule filament system; its destabilizing activity is critical for rapid cytoskeletal remodeling.
• Phosphorylation at Ser-16 has been specifically linked to axon formation during neurogenesis, and stathmin 1 is implicated in the control of both learned and innate fear (by similarity).
• Stathmin 1 is highly expressed in the nervous system (fetal and adult brain, spinal cord, cerebellum) but is found in many tissues; it is particularly overabundant in acute leukemia cells compared to normal leukocytes.
• Subcellular localization is primarily cytoplasmic and associated with the cytoskeleton.
• The protein undergoes multiple post-translational modifications, including phosphorylation, acetylation, and methylation, and alternative splicing generates different isoforms.
• As a developmental and differentiation-related protein, stathmin 1 integrates signals that govern cell morphology, division, and motility.

Experimental Guidance and Technical Tips

• This antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Ser-16 of human stathmin 1; it is expected to recognize the phosphorylated form specifically.
• For western blot, consider treating samples with phosphatase inhibitors to preserve the phosphorylation signal, and validate specificity using phosphatase-treated lysates or blocking with the non-phosphorylated peptide.
• ELISA can be used to quantify phospho-stathmin 1 levels in cell or tissue extracts; use appropriate phosphoprotein standards and ensure linearity within the detection range.
• Since stathmin 1 is a low-molecular-weight protein (17 kDa), run SDS-PAGE with 15% gels or gradient gels for optimal resolution, and use low-fluorescence PVDF membranes for phosphoprotein detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical expertise, and scalable supply solutions—from concept to clinic. The Anti-Phospho-Stathmin 1 (Ser16) antibody supports investigations into microtubule dynamics, neurogenesis, and oncology, particularly in leukemia and brain research. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare-target raw materials to accelerate your assay development and manufacturing workflows.