Anti-Phospho-Stathmin 1 (S25) Rabbit Monoclonal Antibody for WB, ELISA - P16949

Core Product Specifications and Parameters

Biological Background: Phospho-Stathmin 1-S25 Function and Localization

• Stathmin 1 (also known as OP18, p18, p19, metablastin, LAP18, and PR22) is a ubiquitous cytosolic phosphoprotein that functions as a key regulator of microtubule dynamics, promoting microtubule depolymerization and preventing assembly.
• By destabilizing microtubules, stathmin plays critical roles in cell cycle progression, mitosis, and cellular motility, and its phosphorylation at serine 25 (S25) modulates its microtubule‑destabilizing activity.
• Phosphorylation at Ser‑16 is required for axon formation during neurogenesis, and stathmin is involved in the control of learned and innate fear responses.
• Stathmin localizes predominantly to the cytoplasm and associates with the cytoskeleton, consistent with its role in regulating the microtubule filament system.
• Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, and it is overexpressed in acute leukemia cells, underscoring its importance in neural development and oncogenesis.
• The protein undergoes multiple post‑translational modifications, including phosphorylation, acetylation, and methylation, which fine‑tune its activity and interactions.
• Alternative splicing and a coiled‑coil domain contribute to functional diversity and protein‑protein interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Ser25 of human stathmin 1; consider using phosphatase inhibitors during sample preparation to preserve the phospho‑epitope for western blotting.
• For WB, validate the antibody with positive controls such as mitotic cells or okadaic acid‑treated samples where stathmin S25 phosphorylation is induced.
• Reactivity with rat is indicated; however, cross‑reactivity with other species should be verified experimentally.
• For ELISA, assess coating efficiency of the phosphopeptide and include non‑phosphopeptide controls to confirm phosphorylation specificity.
• Due to the ubiquitous expression of stathmin, ensure that loading controls are appropriate for the specific tissue or cell line under investigation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs streamlined access to validated raw materials for biomarker development. This anti‑phospho‑stathmin 1 (S25) antibody supports studies in microtubule biology, neurology, and oncology, complementing our portfolio of optimized phosphorylation‑specific monoclonal antibodies, antigen pairs, and ancillary reagents. For rare‑target needs or bulk supply, our sourcing service delivers customized solutions to accelerate your assay development from concept to clinic.