Anti-Phospho-STAT5A (S780) Rabbit Polyclonal Antibody for WB - P42229

Core Product Specifications and Parameters

Biological Background: STAT5A Function and Localization

• STAT5A is a member of the STAT protein family and functions as both a signal transducer and transcriptional activator in cytokine and growth factor signaling pathways.
• Upon phosphorylation, STAT5A dimerizes and translocates to the nucleus where it binds to the GAS (interferon-gamma activated sequence) element, activating transcription of target genes.
• It mediates cellular responses to cytokines such as KITLG/SCF and growth factors including ERBB4, and potentially activated FGFR1-4 receptors.
• STAT5A plays a critical role in prolactin-induced signaling, regulating milk protein expression during lactation.
• Subcellular localization: Cytoplasm in inactive state; translocates to the nucleus upon activation.
• Post-translational modification: Phosphorylation at serine 780 (S780) is associated with its activation and transcriptional activity.
• Contains an SH2 domain for receptor docking and dimerization, and is subject to ubiquitin conjugation and alternative splicing.
• Keywords: Activator, Alternative splicing, Cytoplasm, DNA-binding, Lactation, Nucleus, Phosphoprotein, SH2 domain, Transcription, Transcription regulation, Ubl conjugation.

Experimental Guidance and Technical Tips

• This antibody is raised against a phospho-specific peptide corresponding to the S780 residue of human STAT5A; it specifically recognizes the phosphorylated form.
• For Western blot, validate the antibody on samples where STAT5A S780 phosphorylation is expected, such as cytokine-stimulated cell lysates. Use appropriate phospho- and total protein controls.
• Rabbit polyclonal antibodies often provide high sensitivity; consider verifying cross-reactivity in your species of interest if other than human.
• If extending applications beyond Western blot (e.g., immunofluorescence), additional validation in your system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This phospho-STAT5A antibody supports research into cell signaling, cancer biology, and lactation. For customized sourcing needs, our team provides one-stop technical services from concept to clinic.