Anti-Phospho-STAT5-Y694 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P42229/P51692

Core Product Specifications and Parameters

Biological Background: STAT5 Function and Localization

• STAT5A and its closely related isoform STAT5B are members of the signal transducer and activator of transcription (STAT) family, acting as dual-function proteins in signal transduction and transcriptional activation.
• Upon phosphorylation at a conserved tyrosine residue (Y694 in STAT5A, Y699 in STAT5B), STAT5 forms dimers that translocate to the nucleus and bind GAS elements to regulate transcription.
• STAT5 mediates cellular responses to a range of cytokines and growth factors, including KITLG/SCF, ERBB4, and activated FGFRs, thereby playing pivotal roles in proliferation, differentiation, and survival.
• In the mammary gland, STAT5 is essential for prolactin-induced transcription, driving the expression of milk proteins during lactation.
• The protein localizes predominantly in the cytoplasm in its inactive state, and undergoes nuclear translocation following phosphorylation.
• STAT5 contains an SH2 domain critical for receptor docking and dimerization, and is subject to various post-translational modifications, including phosphorylation, ubiquitination, and acetylation.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphorylated peptide corresponding to the Y694 region of human STAT5; it is expected to specifically detect phosphorylated STAT5A (and STAT5B) at this site.
• For Western Blot, a band at approximately 90 kDa is anticipated; validate the detection of phosphorylated STAT5 in your cell/tissue lysate by including appropriate positive and negative controls (e.g., stimulated vs. unstimulated samples).
• For IHC-P, consider antigen retrieval methods (e.g., heat-induced epitope retrieval) and validate staining specificity using blocking peptides or phosphatase-treated sections.
• For ELISA, the antibody can be used as either a capture or detection reagent; optimize coating concentrations and detection ranges empirically.
• Note the reported cross-reactivity with mouse and rat; however, individual experiments should confirm suitability for your specific sample type and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials spanning the entire development pipeline—from discovery to commercial production. This Phospho-STAT5-Y694 antibody supports research in cytokine signaling, oncology, and lactation biology. As your dedicated sourcing partner, CamelBio offers validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and custom sourcing for rare targets, helping you accelerate your assay development and scale-up.