Anti-Phospho-STAT5-Y694 Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P42229/P51692

Core Product Specifications and Parameters

Biological Background: STAT5A Function and Localization

• STAT5A (Signal transducer and activator of transcription 5A) is a dual-function protein that mediates signal transduction and activates transcription.
• It transduces signals from cytokines and growth factors, including KITLG/SCF, ERBB4, and activated FGFR1-4.
• Upon phosphorylation at Y694, STAT5A dimerizes and translocates to the nucleus to bind GAS elements, regulating target gene expression.
• Known targets include milk protein genes critical for lactation.
• STAT5A shuttles between the cytoplasm and nucleus; its nuclear localization is enhanced by activation.
• The protein features an SH2 domain, undergoes phosphorylation and ubiquitination, and has alternatively spliced isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide spanning Y694 of human STAT5A/B, ensuring phospho-specific detection.
• For Western blotting, a band around 90 kDa is anticipated; include non-phosphorylated controls to verify specificity.
• In IF and IHC, signal may appear cytoplasmic or nuclear depending on activation status; validate in appropriate cell types or tissues.
• For ELISA, optimize antibody dilution and coating conditions as sensitivity can vary by assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Our expertise in signaling targets like STAT5 supports assay development from concept to clinic. For research or diagnostic needs in the JAK/STAT pathway, CamelBio delivers reliable, one-stop access to quality materials.