Anti-Phospho-STAT3-S727 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P40763

Core Product Specifications and Parameters

Biological Background: STAT3 Function and Localization

• STAT3 (Signal transducer and activator of transcription 3, also known as Acute-phase response factor) mediates cellular responses to interleukins, KITLG/SCF, LEP, and other growth factors (PubMed:10688651, PubMed:11294841, PubMed:12359225). Related references: PMID:10688651 PMID:11294841 PMID:12359225

• Upon activation, it recruits coactivators such as NCOA1 or MED1 to target gene promoters (PubMed:15653507, PubMed:16285960, PubMed:17344214). Related references: PMID:15653507 PMID:16285960 PMID:17344214

• Acts as a critical regulator of inflammatory response by controlling naive CD4(+) T-cell differentiation into Th17 or Treg cells; acetylation promotes its transcriptional activity and cell differentiation (PubMed:28065600, PubMed:28262505). Related references: PMID:28065600 PMID:28262505

• Involved in cell cycle regulation by inducing CCND1 expression, facilitating the G1 to S phase transition (PubMed:17344214). Related references: PMID:17344214

• Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476). Related references: PMID:23084476

• May play an apoptotic role by transactivating BIRC5 expression under LEP activation (PubMed:18242580). Related references: PMID:18242580

• Subcellular localization alternates between cytoplasm and nucleus; phosphorylation at Ser727 and Tyr705 is critical for activation and nuclear import.

• Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, and in naive CD4+ T cells as well as Th17, Th1, and Th2 cells (PubMed:31899195). Related references: PMID:31899195

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Ser727; to confirm phospho-specificity in WB, consider pre-treating samples with phosphatase or using a non-phospho-specific STAT3 antibody for comparison.
• For IHC-P, optimize antigen retrieval methods as they may affect phospho-epitope preservation; validate staining with positive and negative tissue controls.
• Cross-reactivity across human, mouse, and rat suggests conservation of the S727 phosphorylation site; however, always verify specificity in the relevant model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with a comprehensive portfolio of validated IVD raw materials. This anti-Phospho-STAT3-S727 antibody exemplifies our commitment to providing high-quality tools for studying STAT3-mediated signaling pathways. Through our one-stop platform, we offer streamlined access to antibody pairs, bulk ancillary reagents, and rare-target sourcing, helping you accelerate assay development from concept to clinic.