Anti-Phospho-STAT3-S727 Rabbit mAb - P40763

Core Product Specifications and Parameters

Biological Background: STAT3 Function and Localization

• Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP, and other growth factors. Related references: PMID:10688651, PMID:11294841, PMID:12359225
• Upon activation, recruits coactivators (NCOA1/MED1) to target gene promoters to regulate transcription. Related references: PMID:15653507, PMID:16285960, PMID:17344214
• Binds IL6-responsive elements in acute-phase protein gene promoters after IL6 stimulation. Related references: PMID:12359225
• Mediates signaling through FGFR1-4 and IL31. Related references: PMID:12873986, PMID:15194700
• Regulates T-helper cell differentiation: acetylation promotes Th17 differentiation, while deacetylation/oxidation by LOXL3 inhibits it. Related references: PMID:28065600, PMID:28262505
• Involved in cell cycle progression (CCND1 induction), apoptosis (BIRC5 transactivation), and macroautophagy repression (inhibits EIF2AK2/PKR). Related references: PMID:17344214, PMID:18242580, PMID:23084476
• The STAT3 gene undergoes alternative splicing, producing isoforms of approximately 88 kDa and 83 kDa.
• Predominantly cytosolic but translocates to the nucleus upon activation.
• Widely expressed in tissues such as heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas; also found in naive CD4+ T cells and various T-helper subsets. Related references: PMID:31899195

Experimental Guidance and Technical Tips

• For Western blot, expect bands at ~88 kDa and 83 kDa. Use freshly prepared RIPA buffer supplemented with phosphatase inhibitors to preserve the S727 phosphorylation signal.
• When performing immunoprecipitation or ChIP, validate the antibody’s efficiency with appropriate positive (e.g., IL-6‑stimulated cell lysates) and isotype controls.
• In immunofluorescence/immunohistochemistry, observe STAT3 nuclear translocation following cytokine or growth factor treatment as an indicator of pathway activation.
• For ELISA applications, consider pairing with a capture antibody against total STAT3 to build a phospho‑specific sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, including validated antibodies, antigens, and ancillary reagents. This anti‑phospho‑STAT3 (S727) rabbit monoclonal antibody is available through our network, supporting research in JAK/STAT signaling, oncology, and immunology. Whether you need bulk monoclonal antibodies, matched antibody pairs, or rare‑target reagents, CamelBio streamlines your procurement from concept to clinic.