Anti-Phospho-STAT2 (Tyr690) Rabbit Monoclonal Antibody for WB and ELISA - P52630

Core Product Specifications and Parameters

Biological Background: STAT2 Function and Localization

• STAT2 is a signal transducer and activator of transcription that mediates signaling by type I interferons (IFN-α/β). Upon IFN binding, receptor-associated JAKs (TYK2 and JAK1) phosphorylate STAT2 at tyrosine 690, enabling its dimerization with STAT1 and association with IRF9 to form the ISGF3 transcription complex. Related references: PMID:23391734, PMID:9020188
• The ISGF3 complex translocates to the nucleus and binds to the interferon-stimulated response element (ISRE), driving the transcription of interferon-stimulated genes (ISGs) that establish an antiviral cellular state. Related references: PMID:23391734, PMID:9020188
• STAT2 also exerts negative feedback on type I IFN signaling by recruiting USP18 to the IFNAR2 receptor subunit, attenuating the antiviral response. Related references: PMID:28165510
• Beyond its canonical role, STAT2 regulates mitochondrial fission by modulating the phosphorylation of DNM1L at Ser-616 (activating) and Ser-637 (inactivating), impacting mitochondrial dynamics. Related references: PMID:23391734, PMID:26122121
• STAT2 primarily localizes to the cytoplasm but shuttles to the nucleus upon activation.
• The protein contains an SH2 domain critical for phosphotyrosine-mediated interactions and DNA-binding activity, and is subject to ubiquitin conjugation and proteasomal regulation.

Experimental Guidance and Technical Tips

• This antibody specifically recognizes STAT2 phosphorylated at Tyr690; include phosphatase inhibitors during sample preparation to preserve the phosphorylation signal.
• For Western blotting, expect a band at approximately 98 kDa; validate in the relevant human or rat sample system.
• The rabbit monoclonal origin provides high specificity; use appropriate anti-rabbit secondary antibodies for detection.
• For ELISA applications, consider pairing this antibody with a capture or detection reagent after thorough cross-reactivity testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined sourcing platform for high-quality IVD raw materials, including phospho-specific antibodies like this anti-Phospho-STAT2 (Tyr690) mAb. Our one-stop supply covers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target procurement to support your interferon signaling and antiviral research assays.