Anti-Phospho-STAT1 (Y701) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P42224

Core Product Specifications and Parameters

Biological Background: STAT1 Function and Localization

• STAT1 is a signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF, and other growth factors. Related references: PMID:12764129 PMID:12855578 PMID:15322115
• Following type I IFN receptor binding, STAT1 is tyrosine-phosphorylated, dimerizes with STAT2, and associates with IRF9 to form the ISGF3 complex, which binds ISRE to activate antiviral genes. Related references: PMID:28753426 PMID:35568036
• In response to type II IFN (IFN-gamma), STAT1 forms homodimers (GAF) that bind GAS elements to drive target gene expression. Related references: PMID:8156998
• STAT1 is phosphorylated at Tyr701 and Ser727; phosphorylation is critical for its activation and nuclear translocation. Related references: PMID:26479788
• STAT1 also becomes activated in response to KIT signaling through KITLG/SCF. Related references: PMID:15526160
• It may mediate cellular responses to activated FGFR1, FGFR2, FGFR3, and FGFR4. Related references: PMID:19088846
• Upon LPS-induced TLR4 endocytosis, STAT1 is phosphorylated at Thr-749 by IKBKB, promoting transcriptional activation of ARID5A and IL12B. Related references: PMID:32209697
• STAT1 localizes to both the cytoplasm and nucleus, shuttling upon activation. It undergoes various post-translational modifications including acetylation, methylation, ADP-ribosylation, and ubiquitination.

Experimental Guidance and Technical Tips

• This antibody was raised against a phosphopeptide corresponding to the Y701 phosphorylation site of human STAT1. Therefore, it is expected to specifically recognize STAT1 phosphorylated at Tyr701.
• For Western blotting, consider using appropriate phosphatase inhibitors during sample preparation to preserve phosphorylation status.
• In IHC-P, antigen retrieval may be required; validate staining under both phosphorylated and dephosphorylated conditions to confirm specificity.
• Cross-reactivity with mouse and rat STAT1 has been reported, but sequence alignment should be checked for other species before use.
• For ELISA, this antibody may be used as a detection tool; appropriate controls for phosphorylation-specific detection are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials and technical services, covering every step from concept to clinic. This Phospho-STAT1 (Y701) antibody is a valuable tool for studying interferon signaling pathways, and we can assist with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Connect with us to explore how we can accelerate your assay development with reliable, high-quality reagents.