Anti-Phospho-STAT1 (Ser727) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P42224

Core Product Specifications and Parameters

Biological Background: STAT1 Function and Localization

• STAT1 encodes the signal transducer and activator of transcription 1, producing alpha (p91) and beta (p84) isoforms via alternative splicing (UniProt: P42224).
• In type I interferon signaling, STAT1 is phosphorylated and forms the ISGF3 complex with STAT2 and IRF9, binding ISRE elements to activate antiviral gene transcription. Related references: PMID:28753426, PMID:35568036
• In response to type II interferon (IFN-gamma), STAT1 forms homodimers (GAF) that bind GAS elements, driving transcription of immune response genes. Related references: PMID:8156998, PMID:26479788
• STAT1 is activated by various cytokines and growth factors, including KITLG/SCF, and may mediate signals from FGFRs, contributing to diverse cellular responses. Related references: PMID:12764129, PMID:12855578, PMID:15322115, PMID:15526160, PMID:19088846
• LPS-induced TLR4 signaling leads to STAT1 phosphorylation at Thr-749 by IKBKB, promoting binding to ARID5A and IL12B promoters and enhancing pro-inflammatory cytokine expression. Related references: PMID:32209697
• Subcellular localization is predominantly cytoplasmic, with nuclear translocation upon activation; STAT1 is heavily modified by phosphorylation (Tyr701, Ser727), acetylation, methylation, and ubiquitination.
• STAT1 plays a role in food tolerance by associating with cleaved gasdermin-D to drive CIITA transcription and regulatory T cell induction in the small intestine (By similarity).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Ser727; thus, the antibody specifically recognizes STAT1 phosphorylated at Ser727. Ensure sample preparation includes phosphatase inhibitors to preserve phosphorylation.
• For Western blot, use a validated loading control and run lysates from stimulated vs. unstimulated cells to confirm phospho-specificity.
• In IHC-P, consider heat-induced antigen retrieval and validate staining with isotype controls and phosphatase-treated specimens.
• Because cross-reactivity is confirmed in human, mouse, and rat, the antibody is suitable for these species. Validate in the relevant sample system and blocking conditions.

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CamelBio provides diagnostic manufacturers and researchers with integrated access to IVD raw materials, including validated phospho-specific antibodies like this anti-phospho-STAT1 (S727) for immunology and signaling applications. Our portfolio covers antibody pairs, optimized clones, bulk ancillary reagents, and sourcing for hard-to-find targets, supporting every stage from concept to clinic.