Anti-Phospho-STAT1 (S727) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P42224

Core Product Specifications and Parameters

Biological Background: STAT1 Function and Localization

• STAT1 encodes the signal transducer and activator of transcription 1, producing two main isoforms (STAT1α/p91 and STAT1β/p84) through alternative splicing; also known as transcription factor ISGF-3 components p91/p84.
• STAT1 is a key mediator of interferon (IFN) signaling: following type I IFN (IFN-α/β) binding, it forms the ISGF3 complex with STAT2 and IRF9 to activate antiviral genes; in response to type II IFN (IFN-γ), it homodimerizes into GAF and binds GAS elements. Related references: PMID:28753426 PMID:35568036 PMID:26479788 PMID:8156998
• Phosphorylation of STAT1 at Ser727 enhances its transcriptional activity and is critical for full biological responses; this modification occurs in response to type II IFN and other stimuli. Related references: PMID:26479788
• STAT1 also transduces signals from KITLG/SCF and fibroblast growth factor receptors (FGFR1-4). Related references: PMID:15526160 PMID:19088846
• Following LPS-induced TLR4 endocytosis, STAT1 is phosphorylated at Thr-749, leading to transcriptional activation of ARID5A and IL12B. Related references: PMID:32209697
• STAT1 shuttles between the cytoplasm and nucleus; it localizes primarily in the cytoplasm under basal conditions and translocates to the nucleus upon activation.
• STAT1 undergoes extensive post-translational modifications including phosphorylation (Tyr, Ser, Thr), acetylation, ADP-ribosylation, methylation, and ubiquitination, which regulate its activity, interaction, and stability.
• Mutations in STAT1 are associated with immune system disorders and primary immunodeficiencies.

Experimental Guidance and Technical Tips

• This antibody is raised against a synthetic phosphopeptide encompassing Ser727 of human STAT1; ensure that sample preparation includes phosphatase inhibitors to preserve the phosphorylation state.
• For Western blotting, the target protein migrates around 87 kDa; select an appropriate gel percentage (e.g., 8–10% SDS-PAGE) for optimal separation.
• In immunohistochemistry and immunofluorescence, validate staining patterns in known positive control tissues or cell lines, and include phosphatase-treated controls to confirm phospho-specificity.
• The antibody cross-reacts with mouse and rat STAT1, but verify reactivity in the specific experimental system as epitope conservation may vary.
• For ELISA, pair with a validated capture antibody and use synthetic phospho-peptide standards for quantification; carefully optimize blocking and washing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, contract research labs, and academic institutes in sourcing high-quality IVD raw materials. This anti-phospho-STAT1 (S727) antibody is one of many validated reagents available for interferon pathway research. In addition to bulk antibody supply, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal preparations, and custom sourcing services for rare or emerging targets—streamlining your development from discovery to clinical application.