Anti-Phospho-STAT1 (S727) Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P42224

Core Product Specifications and Parameters

Biological Background: STAT1 Function and Localization

• STAT1 (Signal transducer and activator of transcription 1) exists as two alternatively spliced isoforms, STAT1-alpha (p91) and STAT1-beta (p84), which differ in their C-terminal transactivation domain.
• As a key mediator of interferon (IFN) signaling, STAT1 becomes tyrosine-phosphorylated in response to type I IFNs, heterodimerizes with STAT2 and IRF9 to form the ISGF3 complex, and induces antiviral gene expression via ISRE elements. Related references: PMID:28753426, PMID:35568036
• Under type II IFN (IFN-gamma) stimulation, STAT1 is phosphorylated at both tyrosine and serine residues, homodimerizes into GAF, and binds GAS sequences to activate target genes. Related references: PMID:8156998, PMID:26479788
• STAT1 signaling is also triggered by KITLG/SCF and may participate in FGFR-mediated pathways. Related references: PMID:15526160, PMID:19088846
• In innate immunity, TLR4 endocytosis upon LPS stimulation leads to IKBKB-dependent phosphorylation of STAT1 at Thr-749, driving ARID5A and IL12B transcription. Related references: PMID:32209697
• STAT1 is primarily cytoplasmic but undergoes nuclear translocation upon activation; its subcellular shuttling is regulated by phosphorylation and other post-translational modifications, including acetylation, methylation, and ubiquitylation.
• Serine 727 phosphorylation in the transactivation domain is essential for maximal transcriptional activity, and the antibody specifically recognizes this modification, enabling dissection of STAT1 activation dynamics.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Ser727 of human STAT1; ensure sample preparation includes phosphatase inhibitors to preserve the phospho-epitope.
• For Western blot, expect a band near 87 kDa; a non-phosphorylated STAT1 antibody control can help confirm phospho-specificity.
• In immunofluorescence (IF/ICC), optimize fixation and permeabilization to balance antigen accessibility and phospho-epitope retention; methanol fixation may be beneficial.
• For immunohistochemistry (IHC-P), test antigen retrieval conditions carefully; consider using phospho-specific controls such as lambda phosphatase treatment.
• When using ELISA, a phosphopeptide blocking experiment can validate signal specificity.
• Cross-reactivity with mouse and rat STAT1 is listed; nevertheless, verify performance in your specific model system due to potential species-dependent differences in sequence or phosphorylation dynamics.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with comprehensive access to validated IVD antibodies, including phospho-specific STAT1 reagents for JAK/STAT signaling studies. Our one-stop sourcing platform provides antibody pairs, bulk ancillary reagents, and customized raw-material solutions to streamline your assay development from concept to clinic.