Anti-Phospho-Src-Y529 Rabbit Monoclonal Antibody for WB and ELISA - P12931

Core Product Specifications and Parameters

Biological Background: Src Function and Localization

• Src is a proto-oncogene encoding a non-receptor tyrosine kinase activated by various receptors, controlling gene transcription, immune response, cell adhesion, cell cycle, apoptosis, and migration (PubMed:34234773). Related references: PMID:34234773

• In focal adhesions, Src phosphorylates PTK2/FAK1 and paxillin following integrin engagement, regulating cell adhesion and migration (PubMed:21411625). Related references: PMID:21411625

• Src contributes to actin cytoskeleton remodeling via phosphorylation of AFAP1 and cortactin (CTTN).

• SRC mediates EGFR internalization through phosphorylation of clathrin heavy chain (CLTC) at Tyr-1477 (PubMed:18586953). Related references: PMID:18586953

• In osteoclasts, Src is essential for bone resorption by forming a complex with PTK2B/PYK2 and phosphorylating CBL to activate PI3K signaling (PubMed:14585963, PubMed:8755529). Related references: PMID:14585963 PMID:8755529

• Src localizes to the cell membrane, focal adhesions, cytoplasm, cytoskeleton, nucleus, mitochondrial inner membrane, and perinuclear region.

• Expressed ubiquitously with highest levels in platelets, neurons, and osteoclasts; also detected in skin (PubMed:22294297). Related references: PMID:22294297

• Src kinase activity is tightly controlled by phosphorylation, with autophosphorylation at Tyr-419 in the activation loop promoting activity (By similarity).

Experimental Guidance and Technical Tips

• For Western blot, use denaturing and reducing conditions; the expected molecular weight is approximately 60 kDa. Include phosphatase inhibitors during sample preparation to preserve phosphorylation at Y529.
• In ELISA, the phosphorylated peptide immunogen can be employed as the coating antigen, and specificity should be confirmed by parallel testing with a non-phosphorylated control peptide.
• Validate reactivity in human cell lines known to express Src (e.g., HeLa or A549) under conditions that stimulate Y529 phosphorylation, such as serum starvation followed by pervanadate treatment.
• Optimize antibody dilutions empirically for each application; commonly, starting dilutions for WB range from 1:500 to 1:2000, but final working concentrations must be determined by end-users.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents. For studies on Src kinase signaling and its role in cancer or bone biology, we offer this optimized anti-phospho-Src-Y529 rabbit monoclonal antibody. Our sourcing capabilities extend to rare-target reagents and custom development, helping you accelerate assay development from concept to clinic.