Anti-Phospho-Src (S17) Rabbit Polyclonal Antibody for WB and ELISA - P12931

Core Product Specifications and Parameters

Biological Background: Src Function and Localization

• Src is a non-receptor protein tyrosine kinase that regulates diverse cellular activities such as gene transcription, immune response, cell adhesion, cell cycle, apoptosis, and migration. Related references: PMID:34234773
• It phosphorylates focal adhesion proteins like PTK2/FAK1 and paxillin upon integrin engagement, facilitating cytoskeletal reorganization and cell adhesion. Related references: PMID:21411625
• Src plays a critical role in osteoclast-mediated bone resorption by partnering with PTK2B/PYK2 and phosphorylating downstream targets like CBL. Related references: PMID:14585963, PMID:8755529
• The kinase is localized at the cell membrane, mitochondrial inner membrane, nucleus, and cytoskeleton, reflecting its multifunctional signaling roles.
• Multiple isoforms are produced by alternative splicing; isoforms 2 and 3 exhibit higher basal kinase activity compared to canonical isoform 1.
• Src is ubiquitously expressed, with notably high levels in platelets, neurons, and osteoclasts.
• It is a heavily phosphorylated protein with key regulatory sites; this antibody specifically targets phosphorylation at serine 17.
• Keywords: ATP-binding, kinase, SH2/SH3 domains, proto-oncogene, phosphoprotein, and involvement in signaling pathways.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to S17; validate the antibody’s phospho-specificity using phosphatase-treated samples or peptide competition in your assays.
• For Western blotting, a band around 60 kDa is anticipated; adding phosphatase inhibitors to lysis buffers is recommended to preserve phosphorylation.
• In ELISA, this polyclonal antibody may serve as a detection or capture reagent; include unphosphorylated peptide or protein controls to confirm phospho-selectivity.
• Due to multiple Src isoforms, additional bands may appear if other isoforms share the phosphorylated epitope; verify with isoform-specific controls if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic and research applications. For oncology and cell signaling projects targeting Src kinase pathways, we provide reliable sourcing and technical consultation to accelerate your assay development from concept to clinic.