Anti-Phospho-SRC Family (Y416) Rabbit Monoclonal Antibody for WB, ELISA - P12931 / P06241 / P06239 / P07947 / P08631 / P07948

Core Product Specifications and Parameters

Biological Background: SRC Family Kinases Function and Localization

• SRC is a non-receptor protein tyrosine kinase that is activated following engagement of various cellular receptors, including immune receptors, integrins, receptor tyrosine kinases, GPCRs, and cytokine receptors, thereby regulating gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis, migration, and transformation.
  Related references: PMID:34234773

• It belongs to the SRC kinase family, which includes FYN, LCK, YES1, HCK, and LYN; functional redundancy among these members complicates assigning specific roles, but SRC is often the primary kinase activated upon receptor engagement.

• SRC localizes to multiple subcellular compartments including the cell membrane, focal adhesions, adherens junctions, gap junctions, cytoplasm, cytoskeleton, perinuclear region, nucleus, and mitochondrial inner membrane, enabling it to phosphorylate diverse substrates at various cellular locations.

• Phosphorylation of the activation loop tyrosine (Y419 in isoform 1, equivalent to Y416 in the peptide immunogen) relieves autoinhibition and upregulates kinase activity, initiating downstream signaling cascades.

• Ubiquitously expressed, SRC shows 5- to 200-fold higher expression in platelets, neurons, and osteoclasts, where it plays essential roles in hemostasis, neurodevelopment, and bone resorption (osteoclast function requires SRC kinase activity and complex formation with PTK2B/PYK2).
  Related references: PMID:22294297 PMID:14585963 PMID:8755529

• SRC phosphorylates numerous substrates, including focal adhesion kinase (FAK) and paxillin at focal adhesions, coordinating cell adhesion and migration.
  Related references: PMID:21411625

• In addition to its classical roles, SRC participates in antiviral signaling by enhancing RIG-I-mediated responses and facilitates EGFR internalization through clathrin phosphorylation.
  Related references: PMID:19419966 PMID:18586953

Experimental Guidance and Technical Tips

• The immunogen consists of a synthetic phosphorylated peptide surrounding Y416 of the human SRC family, a region highly conserved across SRC family members; this design likely confers pan-SRC family phospho-specificity.
• For western blotting, the predicted molecular weight of SRC and its family members is approximately 60 kDa; verify band specificity using phosphatase-treated lysates or peptide competition assays to confirm phospho-dependence.
• While validated for human samples, cross-reactivity with rat has been observed; users should validate the antibody in their species and tissue of interest, as expression levels and post-translational modifications can vary.
• Consider optimizing antibody dilution and blocking conditions for ELISA to minimize background, particularly when working with complex samples or detecting low-abundance phosphorylated targets.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, supporting diagnostic manufacturers and research laboratories from concept to clinic. This high-quality anti-phospho-SRC family antibody, targeting a conserved activation site, is ideal for kinase signaling and oncology research projects. For custom or rare-target raw material sourcing needs in phospho-signaling pathways, CamelBio leverages its global supply network to deliver reliable solutions tailored to your development workflows.