Anti-Phospho-SQSTM1/p62-S349 Rabbit Polyclonal Antibody for WB, ELISA - Q13501

Core Product Specifications and Parameters

Biological Background: SQSTM1/p62 Function and Localization

• SQSTM1, also known as p62, functions as a molecular adapter for selective macroautophagy (aggrephagy), bridging polyubiquitinated protein aggregates and autophagosomes through its UBA and LIR domains. Related references: PMID:15340068 PMID:15953362 PMID:16286508
• Upon binding to ubiquitinated proteins via the UBA domain, SQSTM1 undergoes liquid-liquid phase separation to form p62 bodies, which are then targeted for autophagic degradation. Related references: PMID:15911346 PMID:20168092 PMID:22017874
• SQSTM1 also serves as a signaling hub, activating the NFE2L2/NRF2 antioxidant response by sequestering KEAP1 into inclusion bodies, thereby promoting nuclear accumulation of NRF2 and cytoprotective gene expression. Related references: PMID:20452972 PMID:28380357 PMID:33393215
• Subcellularly, SQSTM1 localizes to multiple compartments including autophagosomes, cytoplasmic vesicles, PML bodies in the nucleus, late endosomes, lysosomes, and the sarcomere in muscle cells.
• The protein is a known phosphoprotein, with phosphorylation at various residues (including Ser349) affecting its oligomerization, cargo recognition, and autophagy regulation.
• Widely expressed across tissues, SQSTM1 is implicated in neurodegenerative diseases, cancer, and immune signaling.
• It contains several functional domains: PB1 (polymerization), ZZ-type zinc finger, TB (TRAF6-binding), LIR (LC3-interacting region), and UBA (ubiquitin-associated), enabling diverse protein-protein interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide surrounding Ser349, suggesting the antibody specifically recognizes SQSTM1 phosphorylated at this site. Confirm specificity by phosphatase treatment or by comparing signals in stimulated vs. unstimulated cells where appropriate.
• For Western blot analysis, use a reducing SDS-PAGE gel and include fresh phosphatase inhibitors in the lysis buffer to preserve phosphorylation. A band around 48–62 kDa may be observed depending on the sample; SQSTM1 often runs higher due to post-translational modifications.
• For ELISA applications, ELISA assays often require optimization of coating pH and antibody dilutions. Validate the antibody's linear range with known phosphorylated and nonphosphorylated peptide competitors.
• Cross-reactivity with mouse and rat is reported, making it suitable for rodent model studies; always verify in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with validated raw materials for autophagy and cell signaling research, including this phospho-specific SQSTM1 antibody. From monoclonal/polyclonal antibodies to bulk ancillary reagents, we supply every component needed for reliable IVD assay development. Reach out today to explore our sourcing capabilities for rare and custom target reagents.