Anti-Phospho-SMC1 (S957) Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q14683

Core Product Specifications and Parameters

Biological Background: SMC1A Function and Localization

• SMC1A (structural maintenance of chromosomes protein 1A, also known as Sb1.8) is a core component of the cohesin complex, which mediates sister chromatid cohesion after DNA replication to ensure proper chromosome segregation.
• The cohesin complex forms a large ring-like structure that entraps sister chromatids; its cleavage at anaphase triggers chromatid disjunction and mitotic progression.
• SMC1A plays a critical role in DNA repair by interacting with BRCA1 and undergoing phosphorylation by the ATM and ATR kinases, acting as a downstream effector in the S-phase checkpoint.
• The protein localizes to the nucleus, chromosome, centromere, and kinetochore, positioning it at key chromosomal domains for cohesion and repair.
• Post-translational modifications include phosphorylation (e.g., at S957 in response to DNA damage), acetylation, and ubiquitination, which regulate its functions in chromatin dynamics.
• Disease-associated variants in SMC1A are linked to Cornelia de Lange syndrome, epileptic encephalopathy, and intellectual disability, highlighting its importance in development and neurobiology.

Experimental Guidance and Technical Tips

• Because this antibody recognizes a phosphorylation-dependent epitope, always include phosphatase-treated samples or non-phosphorylated peptide competition as negative controls to confirm phospho-specificity.
• For Western blot, consider using mild sample preparation (e.g., without boiling or with gentle reducing conditions) and verify the stability of the phosphorylation signal under your experimental conditions.
• This antibody is validated for WB, IHC-P, IP, and ELISA; titrate and optimize dilutions for each application and validate in the relevant species (human, mouse, rat).
• To induce SMC1A S957 phosphorylation, treat cells with DNA-damaging agents or replication stress inducers, and monitor signal changes as a functional readout.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio supplies a broad portfolio of IVD raw materials, including phospho-specific antibodies, recombinant proteins, and bulk ancillary reagents, to support assay developers from R&D to clinical manufacturing.
• For researchers focused on cohesin biology, DNA damage response, or cell cycle regulation, CamelBio offers access to validated antibody pairs and optimized monoclonal/polyclonal antibodies against key targets like SMC1A.
• As a dedicated B2B partner, CamelBio provides technical consulting and scalable sourcing solutions, ensuring a reliable supply chain for your IVD kit development.