Anti-Phospho-Smad3 (S423/S425) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P84022

Core Product Specifications and Parameters

Biological Background: SMAD3 Function and Localization

• SMAD3 (Mothers against decapentaplegic homolog 3) is a receptor-regulated SMAD (R-SMAD) that functions as an intracellular signal transducer for TGF-beta and activin type 1 receptor kinases.
• Upon phosphorylation at S423/S425, SMAD3 forms complexes with SMAD4 to regulate transcription of target genes by binding the TRE element in promoters, or with SMAD4/JUN/FOS at AP-1/SMAD sites.
• SMAD3 inhibits wound healing by modulating keratinocyte growth and migration and monocyte chemotaxis in a hormone-sensitive manner.
• Positively regulates PDPK1 kinase activity by promoting dissociation from the negative regulator 14-3-3 protein YWHAQ.
• Regulates chondrogenesis and osteogenesis and inhibits early healing of bone fractures.
• Subcellular localization: shuttles between cytoplasm and nucleus; accumulates in the nucleus upon activation.
• Post-translational modifications include phosphorylation, acetylation, ADP-ribosylation, and ubiquitination; interacts with host-virus proteins.

Experimental Guidance and Technical Tips

• The immunogen is a phospho-specific synthetic peptide; always include phosphatase inhibitors during sample preparation to preserve S423/S425 phosphorylation.
• An expected band of approximately 48 kDa is observed in Western blot; confirm migration in your specific gel system.
• Cross-reactivity with mouse and rat is reported; validate detection in the intended species and tissue by including appropriate controls.
• For IHC-P, consider optimizing antigen retrieval and using a phospho-specific detection protocol to ensure signal specificity.
• Confirm phospho-specificity by treating lysates with phosphatase or using non-phosphorylated peptide competition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by providing validated phospho-specific antibodies such as this anti-phospho-Smad3 (S423/S425) antibody, paired with complementary reagents for TGF-beta signaling research. Beyond catalog products, we offer custom antibody development, bulk production, and sourcing of rare-target raw materials, empowering diagnostic manufacturers to accelerate from concept to clinic with confidence.