Anti-Phospho-Smad3 (S423/S425) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P84022

Core Product Specifications and Parameters

Biological Background: Phospho-Smad3 (S423/S425) Function and Localization

• SMAD3 (Mothers against decapentaplegic homolog 3) is a receptor-regulated SMAD (R-SMAD) protein that functions as an intracellular signal transducer for TGF-beta and activin pathways.
• Upon phosphorylation at S423 and S425 by type 1 receptor kinases, SMAD3 forms a complex with SMAD4 and translocates to the nucleus to bind the TRE element, activating transcription of target genes.
• SMAD3 can also form a complex with SMAD4, JUN, and FOS at AP-1/SMAD sites, integrating TGF-beta signaling with other pathways.
• It plays a regulatory role in wound healing, where it appears to inhibit keratinocyte growth and migration, and alters monocyte chemotaxis, with hormone-sensitive effects.
• SMAD3 positively regulates PDPK1 kinase activity by promoting its dissociation from the inhibitory 14-3-3 protein YWHAQ.
• The protein is localized in both the cytoplasm and nucleus, shuttling between compartments in response to signaling.
• Post-translational modifications include phosphorylation, acetylation, ADP-ribosylation, and ubiquitination, with phosphorylation at the C-terminal SSXS motif being critical for activation.

Experimental Guidance and Technical Tips

• For Western blotting, use phospho-specific lysis buffers and include phosphatase inhibitors to maintain S423/S425 phosphorylation.
• Verify band specificity by treating cell lysates with lambda phosphatase or by competing with a non-phosphorylated peptide.
• When performing IHC-P, optimize antigen retrieval conditions and consider phospho-epitope retrieval methods.
• For ELISA, pair with a total SMAD3 antibody to quantify the ratio of phosphorylated to total protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your assay development with a comprehensive portfolio of IVD raw materials. This anti-phospho-Smad3 antibody is part of our offering for cell signaling research, including validated antibody pairs and bulk reagents. We also specialize in sourcing rare targets and customized phospho-specific antibodies to streamline your diagnostic pipeline.