Anti-Phospho-Smad2 (Ser467) Polyclonal Antibody for WB, IHC-P, ELISA - Q15796

Core Product Specifications and Parameters

Biological Background: SMAD2 Function and Localization

• SMAD2 (SMAD family member 2), also known as JV18-1, MADH2, or hMAD-2, is a receptor-regulated SMAD (R-SMAD) that functions as an intracellular signal transducer and transcriptional modulator downstream of TGF-beta and activin type 1 receptor kinases.
• Upon ligand binding, SMAD2 becomes phosphorylated and forms a complex with SMAD4. This complex translocates to the nucleus, binds to the TRE element in target gene promoters, and activates transcription, thereby regulating diverse cellular processes.
• SMAD2 positively regulates PDPK1 kinase activity by promoting its dissociation from the inhibitory 14-3-3 protein YWHAQ, thereby influencing downstream signaling pathways.
• It may act as a tumor suppressor in colorectal carcinoma, as suggested by functional studies. Related references: PMID:8752209
• SMAD2 promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells, highlighting a role in tissue-specific differentiation.
• Subcellular localization is primarily cytoplasmic in the absence of signal, but upon phosphorylation it translocates to the nucleus. The protein shuttles between the cytoplasm and nucleus in a phosphorylation-dependent manner.
• SMAD2 is widely expressed, with high levels found in skeletal muscle, endothelial cells, heart, and placenta, consistent with its role in cardiovascular and developmental signaling.
• The protein undergoes multiple post-translational modifications including phosphorylation (critical for activation), acetylation, and ubiquitination. It is also a zinc-binding phosphoprotein with a molecular weight of approximately 52.3 kDa.

Experimental Guidance and Technical Tips

• The immunogen used was a synthetic phosphorylated peptide around Ser467 of human SMAD2. To ensure detection of the phosphorylated form, consider using phosphatase inhibitors during sample preparation.
• For Western blot (WB), the predicted molecular weight of SMAD2 is ~52 kDa. Observe bands at this size; additional bands may represent isoforms or post-translational modifications.
• In immunohistochemistry (IHC-P), optimize antigen retrieval conditions as phosphorylation-specific antibodies can be sensitive to fixation methods.
• For ELISA, the antibody can be employed for phospho-specific detection; pairing with a total SMAD2 antibody may allow assessment of phosphorylation stoichiometry.
• Validate the antibody's specificity in the relevant species and sample system using appropriate positive and negative controls (e.g., phosphatase-treated lysates for WB).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers, laboratories, and research institutes, offering comprehensive access to IVD raw materials and technical support from concept to clinic. The Anti-Phospho-Smad2 (Ser467) Polyclonal Antibody addresses the growing need for reliable reagents in TGF-beta signaling research and related assay development. We assist clients worldwide in sourcing validated phospho-specific antibodies, bulk ancillary reagents, and rare target raw materials, streamlining their procurement and accelerating assay development workflows.