Anti-Phospho-Smad2-S465/S467 Monoclonal Antibody for WB - Q15796

Core Product Specifications and Parameters

Biological Background: SMAD2 Function and Localization

• SMAD2 (Mothers against decapentaplegic homolog 2) is a receptor-regulated SMAD (R-SMAD) and intracellular signal transducer of the TGF-beta and activin pathways.
• Upon ligand binding, type 1 receptor kinases phosphorylate SMAD2 at S465/S467, enabling complex formation with SMAD4 and nuclear translocation to regulate target gene transcription.
• Alternative names include JV18-1, hMAD-2, MADH2, and MADR2; the protein belongs to the SMAD family and is encoded by the SMAD2 gene.
• SMAD2 binds the TRE promoter element and, with SMAD4, activates TGF-beta-responsive genes involved in processes such as odontoblastic differentiation.
• Acetylation, phosphorylation, and ubiquitination modifications dynamically regulate SMAD2 activity, stability, and subcellular redistribution.
• Expressed at high levels in skeletal muscle, heart, placenta, and endothelial cells.
• May act as a tumor suppressor in colorectal carcinoma.
• Related references: PMID:8752209
• The protein localizes to both the cytoplasm and nucleus, shuttling between compartments upon activation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide spanning S465 and S467 of human SMAD2. To detect endogenous phosphorylation, treat cells with TGF-β1 or activin A and include phosphatase inhibitors in lysis buffers.
• For Western blotting, a band around 52 kDa is expected; validate the signal using phosphatase treatment or non-phospho controls.
• The antibody cross-reacts with human and mouse SMAD2; verify species reactivity in your model system.
• For ELISA, consider coating plates with phospho-peptide and non-phospho-peptide to assess specificity for the phosphorylated epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, specializing in antibodies, antigens, and ancillary reagents for diagnostic assay development. We supply this phospho-SMAD2 antibody as part of our TGF-beta signaling panel, supporting manufacturers and researchers with bulk orders, customized formulations, and technical consultation to accelerate workflow from concept to clinic.