Anti-Phospho-Smad2-S250 Rabbit Monoclonal Antibody for WB, ELISA - Q15796

Core Product Specifications and Parameters

Biological Background: Smad2 Function and Localization

• SMAD2 (also known as JV18-1, MADH2, hMAD-2) is a receptor-regulated SMAD (R-SMAD) and key intracellular signal transducer in the TGF-beta and activin signaling pathways.
• Upon ligand binding, SMAD2 is phosphorylated by type 1 receptor kinases, forms a heteromeric complex with SMAD4, translocates to the nucleus, and binds the TRE element to regulate transcription of target genes.
• SMAD2 positively regulates PDPK1 kinase activity by promoting its dissociation from the inhibitory 14-3-3 protein YWHAQ.
• It may function as a tumor suppressor in colorectal carcinoma. Related references: PMID:8752209
• The protein localizes to both the cytoplasm and nucleus.
• Tissue specificity: Expressed at high levels in skeletal muscle, endothelial cells, heart, and placenta.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination; the protein also contains zinc-binding domains and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to serine 250 of human Smad2; thus, the antibody is specific for the phosphorylated form at this site. Verify specificity using phosphatase treatment or phospho-deficient mutants.
• For Western blot (WB), consider using a total Smad2 antibody as a loading control. Optimize blocking and antibody dilution conditions; BSA-based blocking may reduce background compared to milk when probing for phosphorylated proteins.
• For ELISA, validate in TGF-beta-treated versus untreated cell lysates to confirm activation-dependent detection. Phospho-specific pairs may require careful optimization of capture and detection conditions.
• Listed cross-reactivity includes human and mouse; for other species, check sequence homology at the phosphorylation site and validate experimentally.
• As a rabbit monoclonal antibody, it provides high affinity and consistent lot-to-lot performance, supporting robust assay development.

CamelBio: Your One-Stop Sourcing Bridge

Backed by CamelBio's integrated raw-material supply network, this Phospho-Smad2-S250 antibody is positioned to support diagnostic developers and researchers in TGF-beta pathway studies. CamelBio offers one-stop access to validated antibody pairs, optimized recombinant proteins, and ancillary reagents, streamlining your IVD development from concept to clinic. Explore our portfolio for comprehensive solutions tailored to phosphorylation-specific detection and rare target sourcing needs.