Anti-Phospho-SHP1-Y564 (PTPN6) Polyclonal Antibody for WB, ELISA - P29350

Core Product Specifications and Parameters

Biological Background: SHP-1 (PTPN6) Function and Localization

• SHP-1 (PTPN6) is a non-receptor tyrosine phosphatase, also known as hematopoietic cell protein-tyrosine phosphatase (HCP), PTP-1C, and SH-PTP1. Isoform 1 is expressed in hematopoietic cells, while isoform 2 is found in non-hematopoietic tissues.

• SHP-1 negatively regulates signaling by dephosphorylating receptor tyrosine kinases (RTKs) including EGFR, PDGFR and FGFR, and by acting on immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing receptors such as PDCD1 and LILRB1 to inhibit immune cell activation. Related references: PMID:21258366, PMID:9733788, PMID:11907092, PMID:14739280, PMID:37932456

• It modulates cytokine receptor signaling (e.g., IL-4 receptor) and dephosphorylates cytoplasmic targets such as STING1, LCK, and STAT1, thereby bridging innate and adaptive immune pathways. Related references: PMID:9065461, PMID:34811497, PMID:38532423

• In the nucleus, SHP-1 dephosphorylates transcription factors like NFAT5 and regulates hepatic gluconeogenesis via STAT5A-mediated RNA polymerase II recruitment to the PCK1 promoter. Related references: PMID:37595871

• Subcellularly, SHP-1 localizes to both the cytoplasm and nucleus, consistent with its dual roles in signal termination and transcriptional control.

• The protein is regulated by phosphorylation (e.g., at Y564) and features SH2 domains as well as ubiquitin-like conjugation sites, highlighting its post-translational complexity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Y564; thus, the antibody is expected to specifically recognize the phosphorylated form of SHP-1. Include both untreated and phosphatase-treated controls in western blotting to confirm phospho-specificity.
• For WB, use standard denaturing conditions and consider adding phosphatase inhibitors to the lysis buffer to preserve phosphorylation. The predicted molecular weight is ~68 kDa, but actual migration may vary.
• Validate antibody reactivity in your cell or tissue system of interest, particularly in hematopoietic versus non-hematopoietic sources (isoform expression differences).
• For ELISA, the antibody can be used for phospho-SHP-1 detection; pairing with a total SHP-1 antibody may allow normalization.
• Optimize antibody dilutions and incubation conditions as requirements differ across sample types and experimental designs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents. This phospho-SHP-1 (Y564) rabbit polyclonal antibody is well-suited for immune signaling and cancer studies; our supply network can also source rare-target antibodies and tailor procurement to your development needs. Let CamelBio be your streamlined bridge from concept to clinic.