Anti-Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P62753

Core Product Specifications and Parameters

Biological Background: S6 Ribosomal Protein Function and Localization

• RPS6 (40S ribosomal protein S6) is a component of the 40S small ribosomal subunit and plays a critical role in ribosome assembly and function (PubMed:23636399, PubMed:8706699).
  Related references: PMID:23636399 PMID:8706699

• It controls cell growth and proliferation by regulating the selective translation of specific mRNAs, linking extracellular signals to protein synthesis (PubMed:17220279).
  Related references: PMID:17220279

• RPS6 is part of the small subunit (SSU) processome, a pre-ribosomal complex where it contributes to early rRNA processing and ribosome biogenesis in the nucleolus (PubMed:34516797).
  Related references: PMID:34516797

• Subcellular localization includes the cytoplasm and the nucleus, specifically the nucleolus, reflecting its role in ribosomal subunit maturation.

• The protein is known as phosphoprotein NP33 and is subject to extensive post-translational modifications including phosphorylation, acetylation, ADP-ribosylation, hydroxylation, and ubiquitination (isopeptide bond).

• Phosphorylation of RPS6 occurs at multiple residues, with S235 and S236 being key sites that are targeted by growth factor-stimulated kinases such as p70 S6 kinase, modulating translational activity.

• The protein’s molecular weight is approximately 29 kDa, and it shows high conservation across human, mouse, and rat.

Experimental Guidance and Technical Tips

• Since the antibody recognizes phosphorylation at S235/236, include phosphatase inhibitors in lysis buffers when preparing samples to preserve phosphorylation status.
• For Western blot, consider using a positive control such as cells stimulated with serum or growth factors to induce S6 phosphorylation; the expected band is around 29 kDa.
• This antibody has been validated for WB, IHC-P, and ELISA. For IHC-P, antigen retrieval may be required, and phosphorylation-dependent staining should be validated with appropriate controls.
• Cross-reactivity with mouse and rat has been confirmed; verify performance in your specific experimental system.
• Avoid freeze-thaw cycles and store at recommended conditions to maintain antibody stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and academic institutes with one-stop access to high-quality IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare biological targets. The RPS6 phospho-specific antibody aligns with our portfolio for cell signaling and cancer biomarker development, offering reliable sourcing for immunoassay development. Our team also provides technical consulting and custom supply services to accelerate your project from concept to clinic.