Anti-Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Polyclonal Antibody for WB, ELISA - P62753

Core Product Specifications and Parameters

Biological Background: RPS6 Function and Localization

• RPS6 is a core component of the 40S small ribosomal subunit, essential for ribosome structure and function. Related references: PMID:23636399, PMID:8706699
• It plays a critical role in controlling cell growth and proliferation by selectively translating specific classes of mRNAs. Related references: PMID:17220279
• RPS6 participates in the small subunit (SSU) processome, the first precursor of the small eukaryotic ribosomal subunit, aiding in pre-rRNA folding, modification, and cleavage. Related references: PMID:34516797
• The protein localizes to the cytoplasm, nucleus, and nucleolus, reflecting its dynamic role in ribosome biogenesis and translation.
• RPS6 undergoes multiple post-translational modifications, including phosphorylation, acetylation, ADP-ribosylation, hydroxylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to residues around Ser235 and Ser236 of human RPS6, designed to specifically recognize the phosphorylated form.
• Suitable applications include Western blot (WB) and ELISA; validate the antibody in your specific sample system to confirm reactivity and optimal working conditions.
• Reported cross-reactivity includes human, mouse, and rat; consider verifying in other species if needed.
• For WB, endogenous phosphorylated RPS6 is expected at approximately 29 kDa; optimize antibody dilution and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, from validated antibodies and bulk ancillary reagents to customized sourcing for rare targets. This anti-phospho-S6 ribosomal protein antibody addresses needs in cell growth and translational control research—rely on CamelBio for integrated procurement and technical consulting to accelerate your project.