Anti-Phospho-RPS6KA5-S376 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O75582

Core Product Specifications and Parameters

Biological Background: RPS6KA5 Function and Localization

• RPS6KA5, also known as MSK1 or 90 kDa ribosomal protein S6 kinase 5, is a serine/threonine-protein kinase with a molecular weight of approximately 90 kDa. It belongs to the RSK family and plays critical roles in signal transduction from the cell surface to the nucleus.
• Mediates the mitogen- and stress-induced phosphorylation of transcription factors CREB1 and ATF1, thereby regulating their transcriptional activity. This occurs in response to stimuli such as UV-C, EGF, and anisomycin. Related references: PMID:11909979 PMID:9873047
• Essential for the control of RELA (NF-kB p65) transcriptional activity in response to TNF and glucocorticoids, contributing to the repression of inflammatory gene expression. In skeletal myoblasts, it phosphorylates RELA at Ser-276 during oxidative stress. Related references: PMID:12628924 PMID:18511904
• Directly modulates chromatin structure by phosphorylating histone H3 at Ser-10 and possibly Ser-28, as well as histone H2A at Ser-1. Histone H3 phosphorylation promotes the transcriptional activation of immediate early genes like c-fos and c-jun. Related references: PMID:12773393 PMID:15010469
• Phosphorylates and regulates multiple other substrates, including STAT3 (at Ser-727), the transcription factor ETV1/ER81 (at Ser-191 and Ser-216), the high mobility group protein HMGN1, and the ubiquitin ligase TRIM7 (at Ser-107). Related references: PMID:12763138 PMID:12569367 PMID:25851810
• Localizes to both the nucleus and the cytoplasm, allowing it to transduce signals from cytoplasmic kinase cascades to nuclear targets.
• Widely expressed across tissues, with highest levels in heart, brain, and placenta; lower levels in lung, kidney, and liver.
• Functions in the negative feedback regulation of inflammatory responses in macrophages by limiting pro-inflammatory cytokine production via the TLR4 pathway, likely through induction of DUSP1 and IL10.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to Ser376 of human RPS6KA5. This design likely yields an antibody specific for the phosphorylated form; consider verifying specificity by treating samples with lambda phosphatase or using a non-phosphopeptide competitor in blocking experiments.
• For Western blot (WB) applications, include phosphatase inhibitors in lysis buffers to preserve the phosphorylation signal. The predicted band size is ~90 kDa; confirm against appropriate molecular weight markers.
• In immunohistochemistry (IHC-P), antigen retrieval conditions and detection of phospho-specific epitopes may require optimization. Use known positive control tissues with high RPS6KA5 expression, such as brain or heart.
• For ELISA, the antibody may be used as a capture or detection reagent; pairing with a non-phospho-specific counterpart could allow quantitative assessment of phosphorylation levels. Validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including optimized phospho-specific antibodies like this anti-phospho-RPS6KA5-S376 rabbit pAb. Whether you need validated antibody pairs, bulk ancillary reagents, or assistance sourcing rare targets involved in kinase signaling and inflammatory pathways, our team bridges the gap from concept to clinic. Contact us for tailored procurement solutions that streamline your assay development.