Anti-Phospho-RPS6 (Ser235) Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62753

Core Product Specifications and Parameters

Biological Background: RPS6 Function and Localization

• RPS6, also known as 40S ribosomal protein S6 or Phosphoprotein NP33, is a component of the 40S small ribosomal subunit. It plays an essential role in controlling cell growth and proliferation through the selective translation of specific mRNA classes (PubMed:23636399, PubMed:8706699, PubMed:17220279). Related references: PMID:23636399 PMID:8706699 PMID:17220279

• RPS6 participates in the assembly of the small subunit (SSU) processome, the first precursor of the small eukaryotic ribosomal subunit. Within this complex, it collaborates with ribosome biogenesis factors and an RNA chaperone to process, modify, and cleave pre-ribosomal RNA (PubMed:34516797). Related references: PMID:34516797

• The protein localizes to the cytoplasm, nucleus, and nucleolus, reflecting its roles in both ribosome biogenesis and mature ribosomal function.

• RPS6 is a well-characterized phosphoprotein. Its phosphorylation at Ser235 (and neighboring residues) regulates its activity in translation control. Additional post-translational modifications include acetylation, ADP-ribosylation, hydroxylation, and ubiquitination.

• Keywords associated with RPS6 highlight its roles as a ribonucleoprotein, phosphoprotein, and its involvement in multiple PTM pathways, underscoring its regulatory complexity in growth signaling.

Experimental Guidance and Technical Tips

• The immunogen used was a synthetic phosphopeptide spanning Ser235 of human RPS6. This design targets the phosphorylated epitope, so confirm phospho-specificity in your assay system (e.g., by phosphatase treatment).
• For Western blot (WB), expect a band near 29 kDa. Optimize loading controls and consider phospho‑total normalization.
• In immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), antigen retrieval and permeabilization conditions may affect phospho‑epitope accessibility. Validate with appropriate positive and negative controls.
• When using ELISA, pair this antibody with a suitable capture or detection reagent, ensuring the buffer system preserves the phosphorylation state.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one‑stop partner for IVD raw materials, offering a comprehensive portfolio that includes validated phospho‑specific antibodies like this anti‑Phospho‑RPS6 (S235) mAb, as well as antibody pairs, bulk ancillary reagents, and rare‑target sourcing. We support diagnostic developers and researchers working on cell growth, signaling, and ribosomal biology by streamlining supply chains from concept to clinic. Reach out to explore how our raw‑material solutions can accelerate your project.