Anti-Phospho-RPA2 (T21) Rabbit Monoclonal Antibody for WB, ELISA - P15927

Core Product Specifications and Parameters

Biological Background: RPA2 Function and Localization

• RPA2 (also known as REPA2, RPA32, RP-A p32, RP-A p34) is the 32 kDa subunit of the heterotrimeric replication protein A (RPA) complex, a major single-stranded DNA-binding protein.
• The RPA complex binds and stabilizes ssDNA intermediates formed during DNA replication or upon DNA stress, preventing their reannealing and recruiting proteins involved in DNA metabolism.
• In the cellular response to DNA damage, RPA2 is essential for recruiting the ATRIP-ATR kinase complex and repair factors such as RAD51, RAD52, XPA, XPG, and SMARCAL1 to chromatin.
• RPA2 contributes to nucleotide excision repair (NER), base excision repair (BER) through interaction with UNG, and may also play a role in telomere maintenance.
• RPA stimulates the 5'-3' helicase activity of BRIP1/FANCJ, facilitating replication fork restart and DNA repair. Related references: PMID:17596542
• Subcellular localization is predominantly nuclear, with enrichment in PML bodies.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bond, Ubl conjugation), reflecting its dynamic regulation.
• Alternative splicing generates multiple isoforms, and it is identified as a reference proteome phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around T21 of human RPA2; the antibody is expected to specifically recognize the phosphorylated T21 form. Use appropriate controls (e.g., non-phosphorylated peptide or phosphatase-treated lysates) to confirm specificity.
• For western blot (WB) analysis, the predicted molecular weight of RPA2 is approximately 29 kDa. Include phosphatase inhibitors during sample preparation to preserve phosphorylation.
• The antibody has been applied in WB and ELISA. Optimize dilutions and conditions for your specific experimental system.
• Cross-reactivity is reported with human and rat samples; validate performance in other species if needed.
• Consider using nuclear extracts or DNA-damaged cells to enhance detection, given RPA2's nuclear localization and role in DNA damage response.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This phospho-specific RPA2 (T21) antibody supports research into DNA replication, damage repair, and cancer biology. We offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.