Anti-Phospho-RET (Tyr905) Rabbit Polyclonal Antibody for WB & ELISA - P07949

Core Product Specifications and Parameters

Biological Background: RET Function and Localization

• RET (proto-oncogene tyrosine-protein kinase receptor Ret) is a member of the cadherin superfamily, functioning as a receptor tyrosine kinase that requires both ligand and co-receptor for activation.
  Related references: PMID:21994944, PMID:23333276, PMID:28846097
• It plays essential roles in cell proliferation, neuronal navigation, cell migration, and differentiation, particularly during enteric nervous system and kidney development.
  Related references: PMID:20064382, PMID:20616503, PMID:20702524, PMID:21994944
• RET signaling is triggered by GDNF family ligands binding to GFRα co-receptors, leading to receptor dimerization, autophosphorylation, and activation of downstream MAPK and AKT pathways.
  Related references: PMID:21994944, PMID:23333276, PMID:24560924, PMID:28846097
• RET also functions as a dependence receptor, promoting cell survival in the presence of ligand and inducing apoptosis in its absence; this mechanism is critical in pituitary somatotrophs and sympathetic neurons.
  Related references: PMID:20616503, PMID:21357690
• Subcellularly, RET is localized to the plasma membrane and endosome membrane, where signaling can be sustained or modulated.
• As a phosphoprotein, RET’s kinase activity depends on autophosphorylation of key tyrosine residues, including Tyr905 in the kinase domain, which is essential for catalytic activation and signal transduction.
• Oncogenic and loss-of-function mutations in RET are linked to multiple endocrine neoplasia types 2A/2B (MEN2A, MEN2B) and Hirschsprung disease (HSCR1), underscoring its clinical relevance in neuroendocrine and developmental disorders.

Experimental Guidance and Technical Tips

• Include phosphatase inhibitors in lysis buffers and minimize freeze‑thaw cycles to preserve the phosphorylation signal.
• Use GDNF‑stimulated cell lines (e.g., neuroblastoma or thyroid carcinoma cells expressing RET) as a positive control for western blot detection.
• To verify phospho‑specificity, pre‑incubate the antibody with the phospho‑peptide immunogen; signal should be blocked by the phospho‑peptide but not by the corresponding non‑phosphorylated peptide.
• Due to the large protein size (124 kDa), consider using a gradient or low‑percentage SDS‑PAGE gel and extended transfer times for optimal resolution and transfer efficiency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of high‑quality IVD raw materials, including validated phospho‑specific antibodies such as this anti‑phospho‑RET (Tyr905) antibody, ideally suited for signal transduction research and oncology/developmental disorder assay development. Our one‑stop platform supports diagnostic manufacturers with bulk antibody production, matched antibody pairs, ancillary reagents, and custom sourcing for rare or challenging targets, helping you accelerate the transition from concept to clinical application.