Anti-Phospho-RB (Ser780) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P06400

Core Product Specifications and Parameters

Biological Background: RB1 Function and Localization

• Retinoblastoma-associated protein (RB1) is a tumor suppressor and key regulator of the G1/S transition of the cell cycle. It acts by binding to E2F transcription factors, repressing transcription of genes required for S phase entry. Related references: PMID:10499802
• Phosphorylation of RB1 by cyclin-dependent kinases (CDKs) leads to dissociation from E2F, thereby activating transcription and promoting cell cycle progression. Related references: PMID:10499802
• RB1 also facilitates the G0-G1 transition upon phosphorylation by CDK3/cyclin-C. Related references: PMID:15084261
• Directly involved in heterochromatin formation by maintaining chromatin structure and stabilizing histone methylation, recruiting histone methyltransferases such as SUV39H1.
• Subcellular localization includes the nucleus and cytoplasm, consistent with its roles in transcriptional regulation and cell cycle control.
• Expressed in retina and foreskin keratinocytes, with broad tissue distribution reflecting its fundamental cell cycle function.
• Post-translational modifications include phosphorylation, acetylation, and methylation, which modulate its activity and interactions. Disruption by viral oncoproteins (e.g., SV40 large T antigen, HPV E7) illustrates its central role in growth control.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to residues around Ser780 of human RB. To confirm phospho-specificity, include phosphatase-treated lysates or compete with non-phosphorylated peptide in your assay.
• For Western blot, the predicted molecular weight is approximately 106 kDa under reducing conditions. Use appropriate positive (e.g., cycling cells) and negative (e.g., serum-starved or RB-deficient) controls to verify band identity.
• In IHC-P, antigen retrieval conditions (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) and antibody dilution should be optimized per tissue type. Co-staining with a total RB antibody can help assess phosphorylation status in tissue context.
• The antibody is reported to cross-react with mouse and rat RB; however, validation in the relevant species and experimental system is recommended.
• For ELISA, consider coating with phosphorylated peptide and checking specificity against non-phosphorylated peptide.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. For retinoblastoma protein (RB) and cell cycle research, we provide validated anti-RB antibodies (including phospho-specific clones), recombinant proteins, and bulk ancillary reagents to streamline assay development. Our procurement services extend to rare-target raw materials and custom formulations, ensuring you have one-stop access to critical components for your diagnostic pipelines.