Anti-Phospho-RB (S795) Rabbit Polyclonal Antibody for WB - P06400

Core Product Specifications and Parameters

Biological Background: RB1 Function and Localization

• The RB1 tumor suppressor regulates G1/S cell cycle progression by binding E2F transcription factors, repressing their activity and recruiting chromatin-modifying enzymes (PubMed:10499802). Related references: PMID:10499802
• Cyclin/CDK-mediated phosphorylation of RB1 induces its dissociation from E2Fs, enabling transcription of S-phase genes and promoting cell cycle entry (PubMed:10499802). Related references: PMID:10499802
• RB1 also facilitates the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Related references: PMID:15084261
• Beyond E2F regulation, RB1 maintains heterochromatin structure, recruits methyltransferases SUV39H1, KMT5B, KMT5C, and controls histone H4K20 trimethylation, contributing to epigenetic silencing.
• RB1 interacts with histone deacetylase (HDAC) complexes, such as through SMARCA4/BRG1, to repress promoters like c-FOS in resting neurons.
• In viral infections, SV40 large T antigen, HPV E7, and adenovirus E1A disrupt RB1-E2F complexes, inactivating its tumor suppressor function.
• RB1 is predominantly nuclear, but also found in cytoplasm, and is expressed in retina and foreskin keratinocytes.
• Post-translational modifications include acetylation, phosphorylation, and methylation, which modulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around S795 of human RB1; it may be suitable for detecting phosphorylation-specific signals.
• For Western blot, the predicted molecular weight is 106 kDa; consider a suitable gel percentage and transfer conditions.
• In IHC-P, antigen retrieval and antibody dilution should be optimized for each tissue type.
• Cross-reactivity with mouse and rat suggests utility in those species, but validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials. For RB1 pathway studies, we offer validated phospho-specific and total RB antibodies, matched antibody pairs, and ancillary reagents for streamlined assay development. Contact us for customized sourcing solutions for your next cell cycle oncology project.