Anti-Phospho-RB-S780 Rabbit Monoclonal Antibody for IHC-P, ELISA - P06400

Core Product Specifications and Parameters

Biological Background: Retinoblastoma-associated protein (RB1) Function and Localization

• RB1, or Retinoblastoma-associated protein, is a tumor suppressor that closely governs the G1/S cell cycle checkpoint by binding to and inhibiting E2F transcription factors (PubMed:10499802). Related references: PMID:10499802
• Cyclin-dependent kinase (CDK)-mediated phosphorylation of RB1, including at Ser780, leads to its dissociation from E2F, derepressing genes essential for S-phase entry (PubMed:10499802). Related references: PMID:10499802
• Phosphorylation by CDK3/cyclin‑C also drives the G0‑G1 transition (PubMed:15084261). Related references: PMID:15084261
• RB1 contributes to heterochromatin stability by recruiting histone methyltransferases (SUV39H1, KMT5B/C) and controlling H4K20 trimethylation.
• Subcellularly, RB1 is predominantly nuclear but can shuttle to the cytoplasm; it is expressed in retina and keratinocytes.
• Post‑translational modifications such as phosphorylation, acetylation, and methylation fine‑tune its repressor activity and protein interactions.
• Viral oncoproteins (SV40 large T, HPV E7, adenovirus E1A) disrupt RB1‑E2F complexes, abrogating tumor‑suppressive function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho‑peptide encompassing Ser780 of human RB1. For IHC‑P, consider using antigen retrieval and phosphatase inhibitors to preserve phosphorylation.
• Confirm specificity by comparing staining with a non‑phospho‑specific RB1 antibody or by phosphatase treatment.
• The antibody cross‑reacts with mouse and rat; validate it in the appropriate tissue or cell model for your study.
• For ELISA, include both phosphorylated and unphosphorylated peptide controls to assess phospho‑specificity.
• Because RB1 is a nuclear protein, nuclear enrichment steps may improve detection sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research laboratories with a streamlined procurement pathway for IVD raw materials, including the validated Phospho-RB-S780 Rabbit mAb for cell‑cycle and oncology applications. Leveraging a robust supply network, we offer optimized antibody pairs, bulk recombinant antibodies, and ancillary reagents, and we can source rare‑target raw materials to support your product development from concept to clinic.