Anti-Phospho-Raptor-Ser792 Rabbit Polyclonal Antibody for WB and ELISA - Q8N122

Core Product Specifications and Parameters

Biological Background: Phospho-Raptor (Ser792) Function and Localization

• Raptor (RPTOR), also known as KIAA1303 or RAPTOR, is the regulatory-associated protein of mTOR and a core component of the mTORC1 complex.
• As a key nutrient sensor, mTORC1 promotes anabolic processes (protein, lipid, nucleotide synthesis) and inhibits catabolic pathways (autophagy) by phosphorylating substrates like RPS6KB1 and EIF4EBP1. Related references: PMID:12150925 PMID:12150926 PMID:12747827
• Raptor acts as a molecular adapter within mTORC1, mediating lysosomal recruitment via Rag GTPases and directing TOS motif-containing substrates to the MTOR kinase domain. Related references: PMID:12747827 PMID:24403073 PMID:26588989
• Subcellularly, Raptor localizes to the lysosome membrane, cytoplasm, and cytoplasmic granules, consistent with its signaling role.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination, which regulate mTORC1 activity; the Ser792 residue targeted by this antibody lies within a regulated phosphosite region.
• Tissue expression is broad, with highest levels in skeletal muscle, nasal mucosa, and pituitary, and detectable in brain, lung, kidney, and placenta.
• Alternative splicing generates isoforms, and the protein contains WD repeats that mediate protein-protein interactions.

Experimental Guidance and Technical Tips

• For Western blot analysis, include phosphatase inhibitors in lysis buffers to preserve Ser792 phosphorylation; consider treating cells with mTOR pathway modulators (e.g., rapamycin, amino acid starvation) to assess signal specificity.
• Verify band specificity by antigen competition using the immunizing phosphorylated peptide versus a non-phosphorylated counterpart in your sample system.
• Use a high-percentage transfer gel and extended transfer times for the 149 kDa full-length protein, and always validate in the relevant human or mouse tissue/cell line.
• For ELISA applications, optimize coating conditions and use a phospho-specific detection strategy; cross-reactivity with non-phosphorylated Raptor should be ruled out empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, connecting diagnostic manufacturers and research laboratories with validated, application-tested antibodies like this anti-Phospho-Raptor-Ser792 pAb for mTOR signaling research. Our portfolio includes phospho-specific and total protein antibodies, bulk ancillary reagents, and custom antibody development services, all supported by technical consultation from concept to clinic. Whether you need rare-target reagents, optimized antibody pairs, or scalable production, CamelBio simplifies your supply chain for accelerated assay development.