Anti-Phospho-PTPRA-Y789 Polyclonal Antibody for WB, ELISA - P18433

Core Product Specifications and Parameters

Biological Background: PTPRA Function and Localization

• Encodes receptor-type tyrosine-protein phosphatase alpha (PTPRA), a transmembrane glycoprotein belonging to the protein tyrosine phosphatase (PTP) family.
• Positively regulates integrin-mediated focal adhesion formation, serving as a key modulator of cell-matrix interactions (By similarity).
• Recruits adaptor proteins BCAR3, BCAR1, and CRK to focal adhesions upon integrin engagement, thereby facilitating SRC-mediated phosphorylation cascades that activate PAK and small GTPases RAC1 and CDC42 (By similarity).
• Localizes predominantly to the cell membrane and concentrates at focal adhesions, consistent with its role in adhesion-dependent signaling.
• Undergoes alternative splicing to generate multiple isoforms, potentially contributing to functional diversity.
• Classified as a phosphoprotein, indicating regulation by phosphorylation; the Y789 residue represents a critical regulatory site.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide around Y789; specificity for the phosphorylated epitope should be confirmed by comparing signals from phosphatase-treated versus untreated samples.
• For Western blotting, include phosphatase inhibitors during lysis to preserve phosphorylation. The predicted molecular weight is 91 kDa, but glycosylation and other modifications may shift migration.
• In ELISA applications, consider validating binding using both phosphorylated and non-phosphorylated peptides to assess phospho-specificity.
• The antibody cross-reacts with human, mouse, and rat PTPRA; verify performance in your species and tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers, laboratories, and research institutes from concept to clinic. For studies in integrin signaling and cell adhesion, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing services. Let CamelBio streamline your supply chain with reliable, one-stop access to critical reagents.