Anti-Phospho-PTK2B (Y402) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q14289

Core Product Specifications and Parameters

Biological Background: Protein-tyrosine kinase 2-beta Function and Localization

• Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, migration, adhesion, spreading and bone remodeling.
• Plays a role in the humoral immune response, being required for normal levels of marginal B-cells in the spleen and for migration of splenic B-cells; also regulates macrophage polarization and T-cell responses.
• Promotes osteoclastic bone resorption via cooperation with SRC kinase, and may inhibit osteoprogenitor cell differentiation.
• Functions downstream of a wide range of receptors, including integrin, immune, G protein-coupled, cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress.
• Upon activation, forms signaling complexes with SRC and SRC family kinases, leading to phosphorylation of additional tyrosine residues that create docking sites for scaffold proteins effectors and substrates.
• Activates multiple downstream pathways, including PI3K/AKT1, MAP kinase cascades (ERK, JNK), and Rho family GTPases (RHOA, RAC1), thereby controlling diverse cellular processes.
• Contributes to the regulation of NMDA receptor ion channel activity and intracellular calcium levels by promoting SRC-mediated phosphorylation of NMDA receptors.
• Most abundant in the brain, particularly in amygdala and hippocampus; also expressed in spleen, lymphocytes, and at lower levels in kidney. Subcellularly, it localizes to the cytoplasm, focal adhesions, lamellipodia, cell cortex, and nucleus.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to tyrosine 402 of human PTK2B; specificity for the phosphorylated form should be confirmed using phosphatase treatment or non-phosphorylated blocking peptide controls.
• For Western blotting, include phosphatase inhibitors in lysis buffers to preserve phosphorylation, and consider using a non-phosphorylated PTK2B antibody as a loading control to verify phosphorylation-dependent signal.
• In immunofluorescence/ICC, fixation and permeabilization conditions should be optimized for the cell type; co-staining with a pan-PTK2B antibody may help confirm phospho-specific localization.
• For ELISA applications, the antibody may be used as a capture or detection reagent; validate with phosphorylated and non-phosphorylated peptides to ensure specificity in the assay system.
• The antibody cross-reacts with human, mouse, and rat PTK2B as per supplier data; however, validation in the specific species and experimental context is recommended before quantitative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material procurement, offering a comprehensive portfolio from validated monoclonal and polyclonal antibodies to bulk ancillary reagents. Our experienced team streamlines sourcing for rare and custom targets, ensuring seamless transition from research to clinical application. Whether you are developing assays for integrin signaling, immune cell function, or bone remodeling, CamelBio provides the high-quality antibodies and technical support to accelerate your diagnostic pipeline.