Anti-Phospho-PTEN-S380/T382/383 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P60484

Core Product Specifications and Parameters

Biological Background: PTEN Function and Localization

• PTEN is a dual-specificity protein phosphatase that dephosphorylates tyrosine-, serine-, and threonine-phosphorylated proteins, as well as lipid substrates such as phosphatidylinositol 3,4,5-trisphosphate (PIP3), thereby acting as a key negative regulator of the PI3K-AKT signaling pathway.
  Related references:
  PMID:9187108, PMID:9256433, PMID:9616126
• Lipid phosphatase activity preferentially targets PIP3 to antagonize AKT/PKB signaling, modulating cell cycle progression and survival; tumor suppressor function is linked to this pathway.
  Related references:
  PMID:16824732, PMID:26504226, PMID:9593664, PMID:31492966, PMID:37279284
• Phosphorylation of PTEN at the C‑terminal cluster (including S380, T382, T383) is induced by growth factor stimulation and alters its binding preference from p85 to DLC1, facilitating translocation to the cell posterior and promoting cell migration.
  Related references:
  PMID:26166433
• Dephosphorylates tyrosine-phosphorylated focal adhesion kinase, inhibiting cell migration and integrin-mediated cell spreading and focal adhesion formation.
  Related references:
  PMID:22279049
• Localizes to the cytoplasm, nucleus, PML bodies, dendritic spines, postsynaptic density, and is secreted; the nuclear monoubiquitinated form exhibits stronger pro‑apoptotic activity.
  Related references:
  PMID:10468583, PMID:18716620
• Broadly expressed in adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney, and pancreas; plays roles in synaptic function, adult neurogenesis, and mitochondrial energy metabolism.
• Classified as a tumor suppressor phosphoprotein involved in lipid metabolism, apoptosis, and neurogenesis (Keywords: Acetylation, Alternative splicing, Ubl conjugation, etc.).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide covering the S380, T382, and T383 phosphorylation cluster; the antibody specifically recognizes the phosphorylated form of PTEN. For best results, include phosphatase inhibitors in lysis buffers and consider treating samples with phosphatases (e.g., λ‑phosphatase) to confirm signal specificity.
• In Western blot, the endogenous PTEN band is often observed at ∼54 kDa, although the calculated molecular weight is 47 kDa. The band shift is partly due to phosphorylation; validate the band identity using positive and negative controls (e.g., stimulated vs. unstimulated cells).
• For IHC‑P, standard antigen retrieval techniques (e.g., citrate buffer, high heat) may be required; optimize antibody dilution and incubation conditions for each tissue type.
• This antibody cross‑reacts with human, mouse, and rat PTEN; verify reactivity in your experimental system to ensure species‑specific performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive raw‑material provider for IVD manufacturers and research laboratories, offering end‑to‑end support from concept to commercialization. Our portfolio includes validated phospho‑specific antibodies, optimized monoclonal/polyclonal antibody pairs, and bulk ancillary reagents, ensuring reliable sourcing for PI3K‑AKT pathway and tumor suppressor studies. Leverage CamelBio's global network to streamline procurement of specialized reagents—including this Phospho‑PTEN antibody—and accelerate your diagnostic or research assay development.