Anti-Phospho-PTEN-S380 Rabbit Monoclonal Antibody for WB, ELISA - P60484

Core Product Specifications and Parameters

Biological Background: PTEN Function and Localization

• PTEN (phosphatase and tensin homolog) is a dual-specificity phosphatase that functions as both a lipid and protein phosphatase, and is a well-known tumor suppressor.
• As a lipid phosphatase, it dephosphorylates phosphatidylinositol 3,4,5-trisphosphate (PIP3) to generate PIP2, thereby directly antagonizing the PI3K-AKT signaling pathway and regulating cell survival, proliferation, and metabolism. Related references: PMID:16824732 PMID:26504226 PMID:9593664
• Also exhibits protein phosphatase activity, dephosphorylating tyrosine-, serine-, and threonine-phosphorylated proteins, including focal adhesion kinase, which influences cell migration and adhesion. Related references: PMID:22279049
• PTEN is phosphorylated at multiple residues, including S380, which regulates its activity, conformation, and subcellular localization; phosphorylation at the C-terminal tail reduces its membrane binding and may modulate tumor suppressor function.
• Subcellular localization is dynamic: present in the cytoplasm, nucleus, PML bodies, and at the postsynaptic density in neurons; nuclear monoubiquitinated PTEN exhibits enhanced apoptotic potential. Related references: PMID:10468583 PMID:18716620
• Widely expressed in adult tissues including heart, brain, placenta, lung, liver, muscle, kidney, and pancreas, underscoring its fundamental roles in cellular homeostasis.
• A key modulator of AKT-mTOR signaling during neurogenesis and synaptic plasticity, and required for long-term depression (LTD) in hippocampal neurons.
• Genetic alterations or dysregulation of PTEN are linked to multiple cancers and developmental disorders, highlighting its clinical significance as a therapeutic target.

Experimental Guidance and Technical Tips

• For Western blot, use the antibody at a recommended starting dilution (e.g., 1:500-1:2000) after validation in the experimental system; include phosphatase inhibitors during sample preparation to preserve phosphorylation states.
• In ELISA, confirm optimal coating antigen concentration and antibody dilution through checkerboard titration.
• Validate cross-reactivity with mouse PTEN in appropriate tissue or cell lysates; confirm specificity via peptide competition or treatment with phosphatases.
• Monitor multiple phosphorylation sites to study PTEN regulation interplay; S380 phosphorylation may impact its protein interactions and function.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For PTEN signaling studies, we offer a reliable supply of high-quality phospho-specific and pan antibodies, supporting your research and diagnostic development needs.