Anti-Phospho-PPP2CA/PPP2CB-Y307 Rabbit Polyclonal Antibody for WB, ELISA - P67775 / P62714

Core Product Specifications and Parameters

Biological Background: PPP2CA/PPP2CB Function and Localization

• PPP2CA encodes the alpha isoform of the catalytic subunit of serine/threonine-protein phosphatase 2A (PP2A), a key enzyme regulating numerous signaling pathways and cellular processes.
• PP2A functions as the major phosphatase for microtubule-associated proteins (MAPs) and modulates kinases such as phosphorylase B kinase, casein kinase 2, and MAP-2 kinase (PubMed:22613722). Related references: PMID:22613722
• It dephosphorylates a wide range of substrates including SV40 large T antigen, AXIN1, p53/TP53, PIM3, WEE1, MYC, FOXO3, and AKT1, thereby controlling cell growth, apoptosis, and immune responses (PubMed:10801873, PubMed:12473674, PubMed:17245430, PubMed:25438055, PubMed:30513302). Related references: PMID:10801873, PMID:12473674, PMID:17245430, PMID:25438055, PMID:30513302
• PP2A is a critical component of the STRIPAK complexes, which regulate Hippo, MAPK, nuclear receptor, and cytoskeleton remodeling pathways involved in cell growth and differentiation (PubMed:33633399). Related references: PMID:33633399
• As part of the Integrator-PP2A (INTAC) complex, PPP2CA dephosphorylates the C-terminal domain of RNA polymerase II and SUPT5H/SPT5, mediating premature transcription termination and quality control during elongation (PubMed:33243860, PubMed:34004147, PubMed:37080207). Related references: PMID:33243860, PMID:34004147, PMID:37080207
• It localizes to the cytoplasm, nucleus, centromeres, and spindle poles, reflecting its diverse functions in mitosis, meiosis, and transcriptional regulation.
• The protein is subject to multiple post-translational modifications including phosphorylation, methylation, and ubiquitination, and contains binding sites for iron, manganese, and zinc ions.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic peptide phosphorylated at Y307, ensure that blocking and detection buffers are optimized for phospho-specific antibodies to maintain epitope recognition.
• For Western blot, treat samples with phosphatase inhibitors to preserve the phosphorylation status; include a positive control (e.g., pervanadate-treated cells) to confirm phospho-specificity.
• For ELISA, consider coating with the phosphorylated peptide and using an anti-rabbit secondary antibody for detection. Validate cross-reactivity with mouse samples as indicated.
• When performing WB, expected molecular weight for PPP2CA is approximately 36 kDa; note that PPP2CB may migrate similarly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, offering validated antibodies, bulk reagents, and custom procurement solutions. This phospho-specific anti-PPP2CA/PPP2CB antibody supports signal transduction and cell cycle research, and we can supply matching detection reagents and ancillary materials for your assay development. Contact us to streamline your diagnostic raw-material supply chain from concept to clinic.