Anti-Phospho-PPP1R12A (T853) Rabbit Polyclonal Antibody for WB and ELISA - O14974

Core Product Specifications and Parameters

Biological Background: PPP1R12A Function and Localization

• Encodes protein phosphatase 1 regulatory subunit 12A (PPP1R12A), also known as myosin phosphatase-targeting subunit 1 (MYPT1), myosin-binding subunit (MBS), and GUBS.
• Functions as a key regulator of protein phosphatase 1C (PPP1C), mediating binding to myosin and participating in the dephosphorylation of PLK1 as part of the PPP1C complex.
• Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity, potentially influencing hypoxia-responsive pathways.
• Localizes predominantly to the cytoplasm, with association to the cytoskeleton and stress fibers.
• At the protein level, expressed in striated muscles, specifically in type 2a fibers.
• Belongs to a family of regulatory subunits with ANK repeats and is subject to alternative splicing, hydroxylation, and phosphorylation.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphorylated peptide corresponding to Thr853 of human PPP1R12A. For optimal detection, ensure that samples are prepared under conditions that preserve phosphorylation states (e.g., use phosphatase inhibitors).
• This antibody is suitable for western blot (WB) and ELISA. Consider validating the specific band at the expected molecular weight of 115 kDa in relevant cell or tissue lysates.
• Cross-reactivity has been demonstrated with human, mouse, and rat. When using in other species, verify sequence homology around the phosphorylation site before application.
• As a phospho-specific antibody, it may require optimization of blocking and washing conditions to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw-material sourcing, from concept to clinic. Supporting research into myosin phosphatase signaling and cytoskeletal regulation, this anti-phospho-PPP1R12A (T853) antibody exemplifies the high-quality tools we provide. Whether you need validated antibody pairs, optimized monoclonal or polyclonal antibodies, bulk ancillary reagents, or rare-target raw-material sourcing, CamelBio streamlines your supply chain so you can focus on diagnostic development.