Anti-Phospho-PPP1R12A (S507) Rabbit Polyclonal Antibody for WB, ELISA - O14974

Core Product Specifications and Parameters

Biological Background: PPP1R12A Function and Localization

• PPP1R12A, also known as myosin phosphatase-targeting subunit 1 (MYPT1) or MBS, is the regulatory subunit of protein phosphatase 1 (PP1) and plays a crucial role in cytoskeletal organization and smooth muscle contraction.
• It directs the catalytic subunit PP1C to myosin and other substrates, thereby mediating dephosphorylation of regulatory myosin light chains and also PLK1, influencing mitosis and cell motility.
• The protein contains ankyrin repeats and is subject to alternative splicing, generating isoforms that may exhibit tissue-specific regulatory properties.
• PPP1R12A is predominantly localized in the cytoplasm and associates with the actin cytoskeleton, particularly at stress fibers, consistent with its role in contractile fiber dynamics.
• It is highly expressed in striated muscles, especially in type 2a fast-twitch fibers, where it modulates contraction and relaxation cycles.
• The protein is regulated by phosphorylation at multiple residues, including Ser507, which modulates its inhibitory activity towards PP1C; phosphorylation at this site is associated with signaling pathways involving Rho-kinase.
• Keywords for PPP1R12A include phosphoprotein, alternative splicing, and disease variant, reflecting its regulatory complexity and clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a synthetic phosphopeptide corresponding to residues surrounding Ser507 of human PPP1R12A. For optimal detection, consider using phosphatase inhibitors during sample preparation to preserve the phosphorylation state.
• This antibody has been tested in Western blot (WB) and ELISA applications. For WB, a band at approximately 115 kDa is expected in human cell lines or tissues expressing PPP1R12A. A peptide blocking experiment with the non-phosphorylated counterpart is recommended to confirm phospho-specificity.
• For best results, validate the antibody in your specific sample system, as cross-reactivity with non-specific proteins may occur. Consider using relevant positive control lysates from human smooth muscle or striated muscle tissues.
• This antibody may also be suitable for immunoprecipitation or other phospho-detection assays, though these applications have not been validated; pilot testing is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, recombinant proteins, and ancillary reagents to accelerate diagnostic development. For researchers and manufacturers focusing on myosin phosphatase signaling and smooth muscle pathology, we provide reliable monoclonal and polyclonal antibodies, including bulk options and customized sourcing solutions. Whether you need phospho-specific detection tools or rare-target raw materials, CamelBio bridges your concept to clinic with end-to-end technical support.